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Usefulness of the l‐type Wako Helicobacter pylori antibody J test
BACKGROUND AND AIM: Helicobacter pylori antibody levels in the blood are currently measured using an ELISA. In April 2016, FUJIFILM Wako Pure Chemical Corporation launched the “l‐type Wako Helicobacter pylori antibody J" test, which is based on the latex agglutination turbidimetric immunoassay....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wiley Publishing Asia Pty Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171153/ https://www.ncbi.nlm.nih.gov/pubmed/34124385 http://dx.doi.org/10.1002/jgh3.12553 |
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author | Tokai, Yoshitaka Fujisaki, Junko Ishizuka, Naoki Osumi, Hiroki Namikawa, Ken Yoshimizu, Shoichi Horiuchi, Yusuke Ishiyama, Akiyoshi Yoshio, Toshiyuki Hirasawa, Toshiaki Miki, Kazumasa Tsuchida, Tomohiro |
author_facet | Tokai, Yoshitaka Fujisaki, Junko Ishizuka, Naoki Osumi, Hiroki Namikawa, Ken Yoshimizu, Shoichi Horiuchi, Yusuke Ishiyama, Akiyoshi Yoshio, Toshiyuki Hirasawa, Toshiaki Miki, Kazumasa Tsuchida, Tomohiro |
author_sort | Tokai, Yoshitaka |
collection | PubMed |
description | BACKGROUND AND AIM: Helicobacter pylori antibody levels in the blood are currently measured using an ELISA. In April 2016, FUJIFILM Wako Pure Chemical Corporation launched the “l‐type Wako Helicobacter pylori antibody J" test, which is based on the latex agglutination turbidimetric immunoassay. In this study, we investigated the usefulness of the Wako test. METHODS: We measured H. pylori antibody levels using both the ELISA and Wako test in 180 patients who underwent upper gastrointestinal endoscopy at our hospital between September 2017 and February 2019. Ninety patients were infected with H. pylori. We calculated the diagnostic accuracy, sensitivity, and specificity of each test and the concordance rate between the ELISA and Wako test. If lower limits of 90% confidence intervals (CIs) for each diagnostic validity exceeded the 85% threshold, the usefulness of the diagnostic test was confirmed. RESULTS: The diagnostic accuracy, sensitivity, and specificity were 94.4% (90% CI, 90.8–97.0%), 94.4% (90% CI, 88.7–97.8%), and 94.4% (90% CI, 88.7–97.8%), respectively, when the Wako test was used, and 94.4% (90% CI, 90.8–97.0%), 88.9% (90% CI, 81.9–93.8%), and 100% (90% CI, 96.0–100%), respectively, when the ELISA was used. The concordance rate between the two tests was high (κ = 0.8444). CONCLUSIONS: We confirmed the usefulness of the Wako test, especially when screening for H. pylori infection, due to its high sensitivity. |
format | Online Article Text |
id | pubmed-8171153 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Wiley Publishing Asia Pty Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-81711532021-06-11 Usefulness of the l‐type Wako Helicobacter pylori antibody J test Tokai, Yoshitaka Fujisaki, Junko Ishizuka, Naoki Osumi, Hiroki Namikawa, Ken Yoshimizu, Shoichi Horiuchi, Yusuke Ishiyama, Akiyoshi Yoshio, Toshiyuki Hirasawa, Toshiaki Miki, Kazumasa Tsuchida, Tomohiro JGH Open Original Articles BACKGROUND AND AIM: Helicobacter pylori antibody levels in the blood are currently measured using an ELISA. In April 2016, FUJIFILM Wako Pure Chemical Corporation launched the “l‐type Wako Helicobacter pylori antibody J" test, which is based on the latex agglutination turbidimetric immunoassay. In this study, we investigated the usefulness of the Wako test. METHODS: We measured H. pylori antibody levels using both the ELISA and Wako test in 180 patients who underwent upper gastrointestinal endoscopy at our hospital between September 2017 and February 2019. Ninety patients were infected with H. pylori. We calculated the diagnostic accuracy, sensitivity, and specificity of each test and the concordance rate between the ELISA and Wako test. If lower limits of 90% confidence intervals (CIs) for each diagnostic validity exceeded the 85% threshold, the usefulness of the diagnostic test was confirmed. RESULTS: The diagnostic accuracy, sensitivity, and specificity were 94.4% (90% CI, 90.8–97.0%), 94.4% (90% CI, 88.7–97.8%), and 94.4% (90% CI, 88.7–97.8%), respectively, when the Wako test was used, and 94.4% (90% CI, 90.8–97.0%), 88.9% (90% CI, 81.9–93.8%), and 100% (90% CI, 96.0–100%), respectively, when the ELISA was used. The concordance rate between the two tests was high (κ = 0.8444). CONCLUSIONS: We confirmed the usefulness of the Wako test, especially when screening for H. pylori infection, due to its high sensitivity. Wiley Publishing Asia Pty Ltd 2021-05-07 /pmc/articles/PMC8171153/ /pubmed/34124385 http://dx.doi.org/10.1002/jgh3.12553 Text en © 2021 The Authors. JGH Open: An open access journal of gastroenterology and hepatology published by Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Tokai, Yoshitaka Fujisaki, Junko Ishizuka, Naoki Osumi, Hiroki Namikawa, Ken Yoshimizu, Shoichi Horiuchi, Yusuke Ishiyama, Akiyoshi Yoshio, Toshiyuki Hirasawa, Toshiaki Miki, Kazumasa Tsuchida, Tomohiro Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title | Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title_full | Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title_fullStr | Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title_full_unstemmed | Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title_short | Usefulness of the l‐type Wako Helicobacter pylori antibody J test |
title_sort | usefulness of the l‐type wako helicobacter pylori antibody j test |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171153/ https://www.ncbi.nlm.nih.gov/pubmed/34124385 http://dx.doi.org/10.1002/jgh3.12553 |
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