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Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration

INTRODUCTION: We tested how tube types (ethylenediaminetetraacetic acid [EDTA], serum, lithium heparin [LiHep], and citrate) and freeze–thaw cycles affect levels of blood biomarkers for Alzheimer's disease (AD) pathophysiology, glial activation, and neuronal injury. METHODS: Amyloid beta (Aβ)42...

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Autores principales: Ashton, Nicholas J., Suárez‐Calvet, Marc, Karikari, Thomas K., Lantero‐Rodriguez, Juan, Snellman, Anniina, Sauer, Mathias, Simrén, Joel, Minguillon, Carolina, Fauria, Karine, Blennow, Kaj, Zetterberg, Henrik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171159/
https://www.ncbi.nlm.nih.gov/pubmed/34124336
http://dx.doi.org/10.1002/dad2.12168
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author Ashton, Nicholas J.
Suárez‐Calvet, Marc
Karikari, Thomas K.
Lantero‐Rodriguez, Juan
Snellman, Anniina
Sauer, Mathias
Simrén, Joel
Minguillon, Carolina
Fauria, Karine
Blennow, Kaj
Zetterberg, Henrik
author_facet Ashton, Nicholas J.
Suárez‐Calvet, Marc
Karikari, Thomas K.
Lantero‐Rodriguez, Juan
Snellman, Anniina
Sauer, Mathias
Simrén, Joel
Minguillon, Carolina
Fauria, Karine
Blennow, Kaj
Zetterberg, Henrik
author_sort Ashton, Nicholas J.
collection PubMed
description INTRODUCTION: We tested how tube types (ethylenediaminetetraacetic acid [EDTA], serum, lithium heparin [LiHep], and citrate) and freeze–thaw cycles affect levels of blood biomarkers for Alzheimer's disease (AD) pathophysiology, glial activation, and neuronal injury. METHODS: Amyloid beta (Aβ)42, Aβ40, phosphorylated tau181 (p‐tau181), glial fibrillary acidic protein, total tau (t‐tau), neurofilament light, and phosphorylated neurofilament heavy protein were measured using single molecule arrays. RESULTS: LiHep demonstrated the highest mean value for all biomarkers. Tube types were highly correlated for most biomarkers (r > 0.95) but gave significantly different absolute concentrations. Weaker correlations between tube types were found for Aβ42/40 (r = 0.63–0.86) and serum t‐tau (r = 0.46–0.64). Freeze–thaw cycles highly influenced levels of serum Aβ and t‐tau (P < .0001), and minor decreases in EDTA Aβ40 and EDTA p‐tau181 were found after freeze–thaw cycle 4 (P < .05). DISCUSSION: The same tube type should be used in research studies on blood biomarkers. Individual concentration cut‐offs are needed for each tube type in all tested biomarkers despite being highly correlated. Serum should be avoided for Aβ42, Aβ40, and t‐tau. Freeze–thaw cycles > 3 should be avoided for p‐tau181.
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spelling pubmed-81711592021-06-11 Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration Ashton, Nicholas J. Suárez‐Calvet, Marc Karikari, Thomas K. Lantero‐Rodriguez, Juan Snellman, Anniina Sauer, Mathias Simrén, Joel Minguillon, Carolina Fauria, Karine Blennow, Kaj Zetterberg, Henrik Alzheimers Dement (Amst) Blood‐based Biomarkers INTRODUCTION: We tested how tube types (ethylenediaminetetraacetic acid [EDTA], serum, lithium heparin [LiHep], and citrate) and freeze–thaw cycles affect levels of blood biomarkers for Alzheimer's disease (AD) pathophysiology, glial activation, and neuronal injury. METHODS: Amyloid beta (Aβ)42, Aβ40, phosphorylated tau181 (p‐tau181), glial fibrillary acidic protein, total tau (t‐tau), neurofilament light, and phosphorylated neurofilament heavy protein were measured using single molecule arrays. RESULTS: LiHep demonstrated the highest mean value for all biomarkers. Tube types were highly correlated for most biomarkers (r > 0.95) but gave significantly different absolute concentrations. Weaker correlations between tube types were found for Aβ42/40 (r = 0.63–0.86) and serum t‐tau (r = 0.46–0.64). Freeze–thaw cycles highly influenced levels of serum Aβ and t‐tau (P < .0001), and minor decreases in EDTA Aβ40 and EDTA p‐tau181 were found after freeze–thaw cycle 4 (P < .05). DISCUSSION: The same tube type should be used in research studies on blood biomarkers. Individual concentration cut‐offs are needed for each tube type in all tested biomarkers despite being highly correlated. Serum should be avoided for Aβ42, Aβ40, and t‐tau. Freeze–thaw cycles > 3 should be avoided for p‐tau181. John Wiley and Sons Inc. 2021-06-02 /pmc/articles/PMC8171159/ /pubmed/34124336 http://dx.doi.org/10.1002/dad2.12168 Text en © 2021 The Authors. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring published by Wiley Periodicals, LLC on behalf of Alzheimer's Association https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Blood‐based Biomarkers
Ashton, Nicholas J.
Suárez‐Calvet, Marc
Karikari, Thomas K.
Lantero‐Rodriguez, Juan
Snellman, Anniina
Sauer, Mathias
Simrén, Joel
Minguillon, Carolina
Fauria, Karine
Blennow, Kaj
Zetterberg, Henrik
Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title_full Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title_fullStr Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title_full_unstemmed Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title_short Effects of pre‐analytical procedures on blood biomarkers for Alzheimer's pathophysiology, glial activation, and neurodegeneration
title_sort effects of pre‐analytical procedures on blood biomarkers for alzheimer's pathophysiology, glial activation, and neurodegeneration
topic Blood‐based Biomarkers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171159/
https://www.ncbi.nlm.nih.gov/pubmed/34124336
http://dx.doi.org/10.1002/dad2.12168
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