Cargando…

Observation of liquid–liquid phase separation of ataxin-3 and quantitative evaluation of its concentration in a single droplet using Raman microscopy

Liquid–liquid phase separation (LLPS) plays an important role in a variety of biological processes and is also associated with protein aggregation in neurodegenerative diseases. Quantification of LLPS is necessary to elucidate the mechanism of LLPS and the subsequent aggregation process. In this stu...

Descripción completa

Detalles Bibliográficos
Autores principales:	Murakami, Kazuki, Kajimoto, Shinji, Shibata, Daiki, Kuroi, Kunisato, Fujii, Fumihiko, Nakabayashi, Takakazu
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society of Chemistry 2021
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8171347/ https://www.ncbi.nlm.nih.gov/pubmed/34163831 http://dx.doi.org/10.1039/d0sc06095j

Ejemplares similares

Label-free autofluorescence lifetime reveals the structural dynamics of ataxin-3 inside droplets formed via liquid–liquid phase separation
por: Matsuura, Uchu, et al.
Publicado: (2023)

Pro-Oxidant Activity of an ALS-Linked SOD1 Mutant in Zn-Deficient Form
por: Nagao, Chise, et al.
Publicado: (2020)

SOD1 gains pro-oxidant activity upon aberrant oligomerization: change in enzymatic activity by intramolecular disulfide bond cleavage
por: Yamazaki, Kosuke, et al.
Publicado: (2022)

Single-droplet surface-enhanced Raman scattering decodes the molecular determinants of liquid-liquid phase separation
por: Avni, Anamika, et al.
Publicado: (2022)

Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics
por: Zhang, Sunyuan, et al.
Publicado: (2020)

Measuring the elasticity of liquid–liquid phase separation droplets with biomembrane force probe
por: Sun, Min, et al.
Publicado: (2022)

Watching liquid droplets of TDP-43(CTD) age by Raman spectroscopy
por: Shuster, Sydney O., et al.
Publicado: (2021)

Liquid–Liquid Phase Separation Can Drive Aerosol Droplet Growth in Supersaturated Regimes
por: Malek, Kotiba, et al.
Publicado: (2023)

Liquid–Liquid Phase Separation Induced by Vapor Transfer in Evaporative Binary Sessile Droplets
por: Othman, Ahmed M., et al.
Publicado: (2023)

Study liquid–liquid phase separation with optical microscopy: A methodology review
por: Zhang, Xiufeng, et al.
Publicado: (2023)

Direct imaging of intracellular RNA, DNA, and liquid–liquid phase separated membraneless organelles with Raman microspectroscopy
por: Samuel, Ashok Zachariah, et al.
Publicado: (2022)

A stokes polarimetric light microscopy view of liquid crystal droplets
por: Gou, J., et al.
Publicado: (2021)

DNA Local-Flexibility-Dependent Assembly of Phase-Separated Liquid Droplets
por: Shakya, Anisha, et al.
Publicado: (2018)

Liquid Droplet Microresonators
por: Giorgini, Antonio, et al.
Publicado: (2019)

Liquid-Liquid Phase Separation of Patchy Particles Illuminates Diverse Effects of Regulatory Components on Protein Droplet Formation
por: Nguemaha, Valery, et al.
Publicado: (2018)

Evaporation of a Liquid Droplet
por: Kayser, Richard, et al.
Publicado: (1977)

Liquid Droplets as Emerging Biomaterials
por: Huang, Armando, et al.
Publicado: (2023)

Raman spectroscopy of gases and liquids
por: Weber, Alfons
Publicado: (1979)

Liquid–liquid phase separation in autophagy
por: Noda, Nobuo N., et al.
Publicado: (2020)

RNA and liquid-liquid phase separation
por: Guo, Qi, et al.
Publicado: (2021)

Mass Spectrometry of RNA-Binding Proteins during Liquid–Liquid Phase Separation Reveals Distinct Assembly Mechanisms and Droplet Architectures
por: Sahin, Cagla, et al.
Publicado: (2023)

Simulation and Experiment on Droplet Formation and Separation for Needle-Type Micro-Liquid Jetting Dispenser
por: Lu, Shizhou, et al.
Publicado: (2018)

Smart Superhydrophobic Filter Paper for Water/Oil Separation and Unidirectional Transportation of Liquid Droplet
por: Zhang, Yu-Ping, et al.
Publicado: (2022)

Liquid–Liquid Phase Separation in Crowded Environments
por: André, Alain A. M., et al.
Publicado: (2020)

Targeting viral liquid–liquid phase separation
por: York, Ashley
Publicado: (2021)

Liquid–liquid phase separation in tumor biology
por: Tong, Xuhui, et al.
Publicado: (2022)

Review on Liquid–Liquid Separation by Membrane Filtration
por: Divakar, Swathi, et al.
Publicado: (2022)

Open questions on liquid–liquid phase separation
por: Spruijt, Evan
Publicado: (2023)

Liquid–Liquid Phase Separation in Cardiovascular Diseases
por: Mo, Yuanxi, et al.
Publicado: (2022)

Biomolecular Liquid–Liquid Phase Separation for Biotechnology
por: Shil, Sumit, et al.
Publicado: (2023)

Cantilever-Droplet-Based Sensing of Magnetic Particle Concentrations in Liquids
por: Nyang’au, Wilson Ombati, et al.
Publicado: (2019)

Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin
por: Larson, Adam G., et al.
Publicado: (2017)

Liquid-liquid extraction intensification by micro-droplet rotation in a hydrocyclone
por: Huang, Yuan, et al.
Publicado: (2017)

Stable and Oriented Liquid Crystal Droplets Stabilized by Imidazolium Ionic Liquids
por: Ramou, Efthymia, et al.
Publicado: (2021)

Coalescence Processes of Droplets and Liquid Marbles
por: Jin, Jing, et al.
Publicado: (2017)

Liquid-Metal Enabled Droplet Circuits
por: Ren, Yi, et al.
Publicado: (2018)

DNA-Modified Liquid Crystal Droplets
por: Yang, Xiuxiu, et al.
Publicado: (2022)

Droplet Memory on Liquid-Infused Surfaces
por: Bottone, Davide, et al.
Publicado: (2023)

Rotation and transportation of liquid crystal droplets for visualizing electric properties of microstructured electrodes
por: Bono, Shinji, et al.
Publicado: (2023)

Entropy-driven liquid–liquid separation in supercooled water
por: Holten, V., et al.
Publicado: (2012)

Cannot write session to /tmp/vufind_sessions/sess_jll14njgbppi519lk5uagf1n3n