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Biochemical characterization of a recombinant acid phosphatase from Acinetobacter baumannii

Genomic sequence analysis of Acinetobacter baumannii revealed the presence of a putative Acid Phosphatase (AcpA; EC 3.1.3.2). A plasmid construct was made, and recombinant protein (rAcpA) was expressed in E. coli. PAGE analysis (carried out under denaturing/reducing conditions) of nickel-affinity pu...

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Detalles Bibliográficos
Autores principales:	Smiley-Moreno, Elizabeth, Smith, Douglas, Yu, Jieh-Juen, Cao, Phuong, Arulanandam, Bernard P., Chambers, James P.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2021
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172068/ https://www.ncbi.nlm.nih.gov/pubmed/34077475 http://dx.doi.org/10.1371/journal.pone.0252377

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