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Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway

Phenolic compounds of red wine powder (RWP) extracted from the Italian red wine Aglianico del Vulture have been investigated for the potential immunomodulatory and anti-inflammatory capacity on human macrophages. These compounds reduce the secretion of IL-1β, IL-6, and TNF-α proinflammatory cytokine...

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Autores principales: Santarsiero, Anna, Convertini, Paolo, Vassallo, Antonio, Santoro, Valentina, Todisco, Simona, Iacobazzi, Dominga, Fondufe-Mittendorf, Yvonne, Martelli, Giuseppe, de Oliveira, Marcos R., Montanaro, Rosangela, Brancaleone, Vincenzo, Stöckl, Johannes, Infantino, Vittoria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172326/
https://www.ncbi.nlm.nih.gov/pubmed/34122722
http://dx.doi.org/10.1155/2021/5533793
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author Santarsiero, Anna
Convertini, Paolo
Vassallo, Antonio
Santoro, Valentina
Todisco, Simona
Iacobazzi, Dominga
Fondufe-Mittendorf, Yvonne
Martelli, Giuseppe
de Oliveira, Marcos R.
Montanaro, Rosangela
Brancaleone, Vincenzo
Stöckl, Johannes
Infantino, Vittoria
author_facet Santarsiero, Anna
Convertini, Paolo
Vassallo, Antonio
Santoro, Valentina
Todisco, Simona
Iacobazzi, Dominga
Fondufe-Mittendorf, Yvonne
Martelli, Giuseppe
de Oliveira, Marcos R.
Montanaro, Rosangela
Brancaleone, Vincenzo
Stöckl, Johannes
Infantino, Vittoria
author_sort Santarsiero, Anna
collection PubMed
description Phenolic compounds of red wine powder (RWP) extracted from the Italian red wine Aglianico del Vulture have been investigated for the potential immunomodulatory and anti-inflammatory capacity on human macrophages. These compounds reduce the secretion of IL-1β, IL-6, and TNF-α proinflammatory cytokines and increase the release of IL-10 anti-inflammatory cytokine induced by lipopolysaccharide (LPS). In addition, RWP restores Annexin A1 levels, thus involving activation of proresolutive pathways. Noteworthy, RWP lowers NF-κB protein levels, promoter activity, and nuclear translocation. As a consequence of NF-κB inhibition, reduced promoter activities of SLC25A1—encoding the mitochondrial citrate carrier (CIC)—and ATP citrate lyase (ACLY) metabolic genes have been observed. CIC, ACLY, and citrate are components of the citrate pathway: in LPS-activated macrophages, the mitochondrial citrate is exported by CIC into the cytosol where it is cleaved by ACLY in oxaloacetate and acetyl-CoA, precursors for ROS, NO(·), and PGE(2) inflammatory mediators. We identify the citrate pathway as a RWP target in carrying out its anti-inflammatory activity since RWP reduces CIC and ACLY protein levels, ACLY enzymatic activity, the cytosolic citrate concentration, and in turn ROS, NO(·), PGE(2), and histone acetylation levels. Overall findings suggest that RWP potentially restores macrophage homeostasis by suppressing inflammatory pathways and activating proresolutive processes.
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spelling pubmed-81723262021-06-11 Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway Santarsiero, Anna Convertini, Paolo Vassallo, Antonio Santoro, Valentina Todisco, Simona Iacobazzi, Dominga Fondufe-Mittendorf, Yvonne Martelli, Giuseppe de Oliveira, Marcos R. Montanaro, Rosangela Brancaleone, Vincenzo Stöckl, Johannes Infantino, Vittoria Oxid Med Cell Longev Research Article Phenolic compounds of red wine powder (RWP) extracted from the Italian red wine Aglianico del Vulture have been investigated for the potential immunomodulatory and anti-inflammatory capacity on human macrophages. These compounds reduce the secretion of IL-1β, IL-6, and TNF-α proinflammatory cytokines and increase the release of IL-10 anti-inflammatory cytokine induced by lipopolysaccharide (LPS). In addition, RWP restores Annexin A1 levels, thus involving activation of proresolutive pathways. Noteworthy, RWP lowers NF-κB protein levels, promoter activity, and nuclear translocation. As a consequence of NF-κB inhibition, reduced promoter activities of SLC25A1—encoding the mitochondrial citrate carrier (CIC)—and ATP citrate lyase (ACLY) metabolic genes have been observed. CIC, ACLY, and citrate are components of the citrate pathway: in LPS-activated macrophages, the mitochondrial citrate is exported by CIC into the cytosol where it is cleaved by ACLY in oxaloacetate and acetyl-CoA, precursors for ROS, NO(·), and PGE(2) inflammatory mediators. We identify the citrate pathway as a RWP target in carrying out its anti-inflammatory activity since RWP reduces CIC and ACLY protein levels, ACLY enzymatic activity, the cytosolic citrate concentration, and in turn ROS, NO(·), PGE(2), and histone acetylation levels. Overall findings suggest that RWP potentially restores macrophage homeostasis by suppressing inflammatory pathways and activating proresolutive processes. Hindawi 2021-05-25 /pmc/articles/PMC8172326/ /pubmed/34122722 http://dx.doi.org/10.1155/2021/5533793 Text en Copyright © 2021 Anna Santarsiero et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Santarsiero, Anna
Convertini, Paolo
Vassallo, Antonio
Santoro, Valentina
Todisco, Simona
Iacobazzi, Dominga
Fondufe-Mittendorf, Yvonne
Martelli, Giuseppe
de Oliveira, Marcos R.
Montanaro, Rosangela
Brancaleone, Vincenzo
Stöckl, Johannes
Infantino, Vittoria
Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title_full Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title_fullStr Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title_full_unstemmed Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title_short Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway
title_sort phenolic compounds of red wine aglianico del vulture modulate the functional activity of macrophages via inhibition of nf-κb and the citrate pathway
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172326/
https://www.ncbi.nlm.nih.gov/pubmed/34122722
http://dx.doi.org/10.1155/2021/5533793
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