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Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive

Verticillium wilt of olive (Olea europaea subsp. europaea L.) (VWO), caused by the hemibiotrophic soil-borne fungus Verticillium dahliae Kleb., is considered the major limiting factor of this crop in Mediterranean-type climate regions of the world. The absence of effective chemical treatments makes...

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Autores principales: López-Moral, Ana, Agustí-Brisach, Carlos, Trapero, Antonio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172626/
https://www.ncbi.nlm.nih.gov/pubmed/34093620
http://dx.doi.org/10.3389/fpls.2021.662178
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author López-Moral, Ana
Agustí-Brisach, Carlos
Trapero, Antonio
author_facet López-Moral, Ana
Agustí-Brisach, Carlos
Trapero, Antonio
author_sort López-Moral, Ana
collection PubMed
description Verticillium wilt of olive (Olea europaea subsp. europaea L.) (VWO), caused by the hemibiotrophic soil-borne fungus Verticillium dahliae Kleb., is considered the major limiting factor of this crop in Mediterranean-type climate regions of the world. The absence of effective chemical treatments makes the control of the disease difficult. In this way, the use of biostimulants and host plant defense inducers seems to be one of the most promising biological and eco-friendly alternatives to traditional control measures. Thus, the main goal of this study was to evaluate the effect of 32 products, including amino acids, micronutrients, microorganisms, substances of natural origin, copper complex-based products, and organic and inorganic salts against the disease under controlled conditions. To this end, their effects on mycelial growth and microsclerotia (MS) inhibition of V. dahliae were evaluated by means of dual cultures or by sensitivity tests in vitro as well as on disease progression in planta. Wide ranging responses to the pathogen and disease reduction levels were observed among all the products tested, suggesting multiple modes of action. Copper-based products were among the most effective for mycelial growth and MS inhibition, whereas they did not show an important effect on the reduction of disease severity in planta. Phoma sp. and Aureobasidium pullulans were the most effective in disease reduction in planta with foliar application. On the other hand, two phosphite salts, one with copper and the other with potassium, were the most effective in disease reduction in planta when they were applied by irrigation, followed by A. pullulans and Bacillus amyloliquefaciens. This study will be useful to select the best candidates for future studies, contributing significantly to new insights into the current challenge of the biological control of VWO.
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spelling pubmed-81726262021-06-04 Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive López-Moral, Ana Agustí-Brisach, Carlos Trapero, Antonio Front Plant Sci Plant Science Verticillium wilt of olive (Olea europaea subsp. europaea L.) (VWO), caused by the hemibiotrophic soil-borne fungus Verticillium dahliae Kleb., is considered the major limiting factor of this crop in Mediterranean-type climate regions of the world. The absence of effective chemical treatments makes the control of the disease difficult. In this way, the use of biostimulants and host plant defense inducers seems to be one of the most promising biological and eco-friendly alternatives to traditional control measures. Thus, the main goal of this study was to evaluate the effect of 32 products, including amino acids, micronutrients, microorganisms, substances of natural origin, copper complex-based products, and organic and inorganic salts against the disease under controlled conditions. To this end, their effects on mycelial growth and microsclerotia (MS) inhibition of V. dahliae were evaluated by means of dual cultures or by sensitivity tests in vitro as well as on disease progression in planta. Wide ranging responses to the pathogen and disease reduction levels were observed among all the products tested, suggesting multiple modes of action. Copper-based products were among the most effective for mycelial growth and MS inhibition, whereas they did not show an important effect on the reduction of disease severity in planta. Phoma sp. and Aureobasidium pullulans were the most effective in disease reduction in planta with foliar application. On the other hand, two phosphite salts, one with copper and the other with potassium, were the most effective in disease reduction in planta when they were applied by irrigation, followed by A. pullulans and Bacillus amyloliquefaciens. This study will be useful to select the best candidates for future studies, contributing significantly to new insights into the current challenge of the biological control of VWO. Frontiers Media S.A. 2021-05-20 /pmc/articles/PMC8172626/ /pubmed/34093620 http://dx.doi.org/10.3389/fpls.2021.662178 Text en Copyright © 2021 López-Moral, Agustí-Brisach and Trapero. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
López-Moral, Ana
Agustí-Brisach, Carlos
Trapero, Antonio
Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title_full Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title_fullStr Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title_full_unstemmed Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title_short Plant Biostimulants: New Insights Into the Biological Control of Verticillium Wilt of Olive
title_sort plant biostimulants: new insights into the biological control of verticillium wilt of olive
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172626/
https://www.ncbi.nlm.nih.gov/pubmed/34093620
http://dx.doi.org/10.3389/fpls.2021.662178
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