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MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo

Lung cancer is one of the central causes of tumor-related deaths globally, of which non-small cell lung cancer (NSCLC) takes up about 85%. As key regulators of various biological processes, microRNAs (miRNAs) have been verified as crucial factors in NSCLC. To elucidate the role of miR-486-5p in the...

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Autores principales: Ding, Lei, Tian, Wu, Zhang, Hui, Li, Wanqiu, Ji, Chunyu, Wang, Yuanyuan, Li, Yanli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172781/
https://www.ncbi.nlm.nih.gov/pubmed/34094949
http://dx.doi.org/10.3389/fonc.2021.655236
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author Ding, Lei
Tian, Wu
Zhang, Hui
Li, Wanqiu
Ji, Chunyu
Wang, Yuanyuan
Li, Yanli
author_facet Ding, Lei
Tian, Wu
Zhang, Hui
Li, Wanqiu
Ji, Chunyu
Wang, Yuanyuan
Li, Yanli
author_sort Ding, Lei
collection PubMed
description Lung cancer is one of the central causes of tumor-related deaths globally, of which non-small cell lung cancer (NSCLC) takes up about 85%. As key regulators of various biological processes, microRNAs (miRNAs) have been verified as crucial factors in NSCLC. To elucidate the role of miR-486-5p in the mTOR pathway, we investigated its role in NSCLC and related signaling. Our results confirmed that miR-486-5p was downregulated in most of human NSCLC tissue samples and cell lines. Further study confirmed that it inhibited NSCLC through repression of the mTOR pathway via targeting both ribosomal proteins S6 kinase A1 (RPS6KA1, RSK) and ribosomal proteins S6 kinase B1 (RPS6KB1, p70S6K), which are critical components of the mTOR signaling. Additionally, miR-486-5p impeded tumor growth in vivo and inhibited tumor metastasis through repression of the epithelial-mesenchymal transition (EMT). Taken together, our study verified the role that miR-486-5p exerts in NSCLC, and its expression pattern in the different stages and morphologies of NSCLC makes it a promising biomarker in the early diagnosis of the disease.
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spelling pubmed-81727812021-06-04 MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo Ding, Lei Tian, Wu Zhang, Hui Li, Wanqiu Ji, Chunyu Wang, Yuanyuan Li, Yanli Front Oncol Oncology Lung cancer is one of the central causes of tumor-related deaths globally, of which non-small cell lung cancer (NSCLC) takes up about 85%. As key regulators of various biological processes, microRNAs (miRNAs) have been verified as crucial factors in NSCLC. To elucidate the role of miR-486-5p in the mTOR pathway, we investigated its role in NSCLC and related signaling. Our results confirmed that miR-486-5p was downregulated in most of human NSCLC tissue samples and cell lines. Further study confirmed that it inhibited NSCLC through repression of the mTOR pathway via targeting both ribosomal proteins S6 kinase A1 (RPS6KA1, RSK) and ribosomal proteins S6 kinase B1 (RPS6KB1, p70S6K), which are critical components of the mTOR signaling. Additionally, miR-486-5p impeded tumor growth in vivo and inhibited tumor metastasis through repression of the epithelial-mesenchymal transition (EMT). Taken together, our study verified the role that miR-486-5p exerts in NSCLC, and its expression pattern in the different stages and morphologies of NSCLC makes it a promising biomarker in the early diagnosis of the disease. Frontiers Media S.A. 2021-05-20 /pmc/articles/PMC8172781/ /pubmed/34094949 http://dx.doi.org/10.3389/fonc.2021.655236 Text en Copyright © 2021 Ding, Tian, Zhang, Li, Ji, Wang and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Ding, Lei
Tian, Wu
Zhang, Hui
Li, Wanqiu
Ji, Chunyu
Wang, Yuanyuan
Li, Yanli
MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title_full MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title_fullStr MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title_full_unstemmed MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title_short MicroRNA-486-5p Suppresses Lung Cancer via Downregulating mTOR Signaling In Vitro and In Vivo
title_sort microrna-486-5p suppresses lung cancer via downregulating mtor signaling in vitro and in vivo
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172781/
https://www.ncbi.nlm.nih.gov/pubmed/34094949
http://dx.doi.org/10.3389/fonc.2021.655236
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