Cargando…
Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus
Enterovirus 71 (EV71) is a neurotropic pathogen that causes hand, foot, and mouth disease (HFMD) and it has been consistently associated with severe neurological, cardiac, and respiratory complications. Yet there is no specific treatment for this virus and we still know little about the viral pathog...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172962/ https://www.ncbi.nlm.nih.gov/pubmed/34093481 http://dx.doi.org/10.3389/fmicb.2021.665879 |
_version_ | 1783702622056742912 |
---|---|
author | Yang, Hang Zhao, Xiaohui Xun, Meng Ma, Chaofeng Wang, Hongliang |
author_facet | Yang, Hang Zhao, Xiaohui Xun, Meng Ma, Chaofeng Wang, Hongliang |
author_sort | Yang, Hang |
collection | PubMed |
description | Enterovirus 71 (EV71) is a neurotropic pathogen that causes hand, foot, and mouth disease (HFMD) and it has been consistently associated with severe neurological, cardiac, and respiratory complications. Yet there is no specific treatment for this virus and we still know little about the viral pathogenesis. In this study, we first generated an infectious cDNA clone of EV71 virus from a patient virus strain and made a full-length virus with a NanoLuc reporter gene through reverse genetic approaches. The reporter gene of this virus is genetically stable when passaging in cells and could be used for antiviral testing. In addition, we also made subgenomic replicons (SGRs) of EV71, which lacks part of the structural genes dispensable for viral replication and showed that SGR can be used for viral replication study. Overall, these reporter viral systems are useful tools for EV71 pathogenesis study and antiviral screening. |
format | Online Article Text |
id | pubmed-8172962 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81729622021-06-04 Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus Yang, Hang Zhao, Xiaohui Xun, Meng Ma, Chaofeng Wang, Hongliang Front Microbiol Microbiology Enterovirus 71 (EV71) is a neurotropic pathogen that causes hand, foot, and mouth disease (HFMD) and it has been consistently associated with severe neurological, cardiac, and respiratory complications. Yet there is no specific treatment for this virus and we still know little about the viral pathogenesis. In this study, we first generated an infectious cDNA clone of EV71 virus from a patient virus strain and made a full-length virus with a NanoLuc reporter gene through reverse genetic approaches. The reporter gene of this virus is genetically stable when passaging in cells and could be used for antiviral testing. In addition, we also made subgenomic replicons (SGRs) of EV71, which lacks part of the structural genes dispensable for viral replication and showed that SGR can be used for viral replication study. Overall, these reporter viral systems are useful tools for EV71 pathogenesis study and antiviral screening. Frontiers Media S.A. 2021-05-20 /pmc/articles/PMC8172962/ /pubmed/34093481 http://dx.doi.org/10.3389/fmicb.2021.665879 Text en Copyright © 2021 Yang, Zhao, Xun, Ma and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Yang, Hang Zhao, Xiaohui Xun, Meng Ma, Chaofeng Wang, Hongliang Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title | Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title_full | Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title_fullStr | Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title_full_unstemmed | Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title_short | Reverse Genetic Approaches for the Generation of Full Length and Subgenomic Replicon of EV71 Virus |
title_sort | reverse genetic approaches for the generation of full length and subgenomic replicon of ev71 virus |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8172962/ https://www.ncbi.nlm.nih.gov/pubmed/34093481 http://dx.doi.org/10.3389/fmicb.2021.665879 |
work_keys_str_mv | AT yanghang reversegeneticapproachesforthegenerationoffulllengthandsubgenomicrepliconofev71virus AT zhaoxiaohui reversegeneticapproachesforthegenerationoffulllengthandsubgenomicrepliconofev71virus AT xunmeng reversegeneticapproachesforthegenerationoffulllengthandsubgenomicrepliconofev71virus AT machaofeng reversegeneticapproachesforthegenerationoffulllengthandsubgenomicrepliconofev71virus AT wanghongliang reversegeneticapproachesforthegenerationoffulllengthandsubgenomicrepliconofev71virus |