An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing

In microbiome research, phylogenetic and functional marker gene amplicon sequencing is the most commonly-used community profiling approach. Consequently, a plethora of protocols for the preparation and multiplexing of samples for amplicon sequencing have been developed. Here, we present two economic...

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Detalles Bibliográficos
Autores principales: Pjevac, Petra, Hausmann, Bela, Schwarz, Jasmin, Kohl, Gudrun, Herbold, Craig W., Loy, Alexander, Berry, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173057/
https://www.ncbi.nlm.nih.gov/pubmed/34093488
http://dx.doi.org/10.3389/fmicb.2021.669776

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173057/
https://www.ncbi.nlm.nih.gov/pubmed/34093488
http://dx.doi.org/10.3389/fmicb.2021.669776

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