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An Economical and Flexible Dual Barcoding, Two-Step PCR Approach for Highly Multiplexed Amplicon Sequencing
In microbiome research, phylogenetic and functional marker gene amplicon sequencing is the most commonly-used community profiling approach. Consequently, a plethora of protocols for the preparation and multiplexing of samples for amplicon sequencing have been developed. Here, we present two economic...
Autores principales: | Pjevac, Petra, Hausmann, Bela, Schwarz, Jasmin, Kohl, Gudrun, Herbold, Craig W., Loy, Alexander, Berry, David |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173057/ https://www.ncbi.nlm.nih.gov/pubmed/34093488 http://dx.doi.org/10.3389/fmicb.2021.669776 |
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