Cargando…

Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway

P2X7/NLRP1/caspase-1 mediated neuronal injury plays an important role in diabetic cognitive impairment and eventually inflammatory cascade reaction. Chinese herbal compound Naofucong has been mainly used to treat cognitive disorders in Traditional Chinese Medicine The present study aimed to investig...

Descripción completa

Detalles Bibliográficos
Autores principales: Jing, Guangchan, Wang, Huanyuan, Nan, Fengwei, Liu, Yuqin, Zhang, Mengren
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173084/
https://www.ncbi.nlm.nih.gov/pubmed/34093185
http://dx.doi.org/10.3389/fphar.2021.647116
_version_ 1783702650288603136
author Jing, Guangchan
Wang, Huanyuan
Nan, Fengwei
Liu, Yuqin
Zhang, Mengren
author_facet Jing, Guangchan
Wang, Huanyuan
Nan, Fengwei
Liu, Yuqin
Zhang, Mengren
author_sort Jing, Guangchan
collection PubMed
description P2X7/NLRP1/caspase-1 mediated neuronal injury plays an important role in diabetic cognitive impairment and eventually inflammatory cascade reaction. Chinese herbal compound Naofucong has been mainly used to treat cognitive disorders in Traditional Chinese Medicine The present study aimed to investigate whether its neuroprotective effects might be related to the inhibition of P2X7R/NLRP1/caspase-1 mediated neuronal injury or not. In this study, high glucose-induced HT22 hippocampal neurons were used to determine Naofucong-containing serum neuronal protective effects. Lentiviruses knock out of TXNIP and P2X7R was used to determine that protective effects of Naofucong was related to inflammatory response and P2X7/NLRP1/caspase-1 mediated neuronal injury. NAC was also used to inhibit oxidative stress, so as to determine that oxidative stress is an important starting factor for neuronal injury of HT22 cells cultured with high glucose. Naofucong decreased apoptosis, IL-1β and IL-18 levels in high glucose-induced HT22 hippocampal neuron cells. Naofucong suppressed NLRP1/caspase-1 mediated neuronal injury, and P2X7 was involved in process. HT22 cells cultured in high glucose had an internal environment with elevated oxidative stress, which could promote neuronal injury. The current study demonstrated that Naofucong could significantly improve high glucose-induced HT22 hippocampal neuron injury, which might be related to suppress P2X7R/NLRP1/caspase-1 pathway, which provides novel evidence to support the future clinical use of Naofucong.
format Online
Article
Text
id pubmed-8173084
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81730842021-06-04 Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway Jing, Guangchan Wang, Huanyuan Nan, Fengwei Liu, Yuqin Zhang, Mengren Front Pharmacol Pharmacology P2X7/NLRP1/caspase-1 mediated neuronal injury plays an important role in diabetic cognitive impairment and eventually inflammatory cascade reaction. Chinese herbal compound Naofucong has been mainly used to treat cognitive disorders in Traditional Chinese Medicine The present study aimed to investigate whether its neuroprotective effects might be related to the inhibition of P2X7R/NLRP1/caspase-1 mediated neuronal injury or not. In this study, high glucose-induced HT22 hippocampal neurons were used to determine Naofucong-containing serum neuronal protective effects. Lentiviruses knock out of TXNIP and P2X7R was used to determine that protective effects of Naofucong was related to inflammatory response and P2X7/NLRP1/caspase-1 mediated neuronal injury. NAC was also used to inhibit oxidative stress, so as to determine that oxidative stress is an important starting factor for neuronal injury of HT22 cells cultured with high glucose. Naofucong decreased apoptosis, IL-1β and IL-18 levels in high glucose-induced HT22 hippocampal neuron cells. Naofucong suppressed NLRP1/caspase-1 mediated neuronal injury, and P2X7 was involved in process. HT22 cells cultured in high glucose had an internal environment with elevated oxidative stress, which could promote neuronal injury. The current study demonstrated that Naofucong could significantly improve high glucose-induced HT22 hippocampal neuron injury, which might be related to suppress P2X7R/NLRP1/caspase-1 pathway, which provides novel evidence to support the future clinical use of Naofucong. Frontiers Media S.A. 2021-05-20 /pmc/articles/PMC8173084/ /pubmed/34093185 http://dx.doi.org/10.3389/fphar.2021.647116 Text en Copyright © 2021 Jing, Wang, Nan, Liu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Jing, Guangchan
Wang, Huanyuan
Nan, Fengwei
Liu, Yuqin
Zhang, Mengren
Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title_full Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title_fullStr Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title_full_unstemmed Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title_short Naofucong Ameliorates High Glucose Induced Hippocampal Neuron Injury Through Suppressing P2X7/NLRP1/Caspase-1 Pathway
title_sort naofucong ameliorates high glucose induced hippocampal neuron injury through suppressing p2x7/nlrp1/caspase-1 pathway
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173084/
https://www.ncbi.nlm.nih.gov/pubmed/34093185
http://dx.doi.org/10.3389/fphar.2021.647116
work_keys_str_mv AT jingguangchan naofucongameliorateshighglucoseinducedhippocampalneuroninjurythroughsuppressingp2x7nlrp1caspase1pathway
AT wanghuanyuan naofucongameliorateshighglucoseinducedhippocampalneuroninjurythroughsuppressingp2x7nlrp1caspase1pathway
AT nanfengwei naofucongameliorateshighglucoseinducedhippocampalneuroninjurythroughsuppressingp2x7nlrp1caspase1pathway
AT liuyuqin naofucongameliorateshighglucoseinducedhippocampalneuroninjurythroughsuppressingp2x7nlrp1caspase1pathway
AT zhangmengren naofucongameliorateshighglucoseinducedhippocampalneuroninjurythroughsuppressingp2x7nlrp1caspase1pathway