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Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis

An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work...

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Autores principales: Peralta, Ma. Florencia, Guzman, Ma. Laura, Bracamonte, Ma. Estefanía, Marco, J. Diego, Olivera, Ma. Eugenia, Carrer, Dolores C., Barroso, Paola A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173260/
https://www.ncbi.nlm.nih.gov/pubmed/34124403
http://dx.doi.org/10.1016/j.heliyon.2021.e07136
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author Peralta, Ma. Florencia
Guzman, Ma. Laura
Bracamonte, Ma. Estefanía
Marco, J. Diego
Olivera, Ma. Eugenia
Carrer, Dolores C.
Barroso, Paola A.
author_facet Peralta, Ma. Florencia
Guzman, Ma. Laura
Bracamonte, Ma. Estefanía
Marco, J. Diego
Olivera, Ma. Eugenia
Carrer, Dolores C.
Barroso, Paola A.
author_sort Peralta, Ma. Florencia
collection PubMed
description An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work was to investigate the in vivo efficacy of topical Ris and EuE-Ris complexes on CL. Surface charge and Ris release kinetics from the different dispersions were analyzed. BALB/c mice were infected intradermally with promastigotes of L. (L.) amazonensis. Ulcers were treated with Ris or EuE-Ris hydrogels. All the lesions that received topical Ris or EuE-Ris showed an improvement with respect to control: reduction of ulcer average size, cicatrization, flattened edges and no signs of necrosis. In addition, a marked parasitic inhibition of 69.5 and 73.7% was observed in the groups treated with Ris and EuE-Ris, respectively, with the IgG2a levels indicating a tendency towards cure. The results are promising and the system should now be enhanced to achieve total parasite elimination.
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spelling pubmed-81732602021-06-11 Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis Peralta, Ma. Florencia Guzman, Ma. Laura Bracamonte, Ma. Estefanía Marco, J. Diego Olivera, Ma. Eugenia Carrer, Dolores C. Barroso, Paola A. Heliyon Research Article An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work was to investigate the in vivo efficacy of topical Ris and EuE-Ris complexes on CL. Surface charge and Ris release kinetics from the different dispersions were analyzed. BALB/c mice were infected intradermally with promastigotes of L. (L.) amazonensis. Ulcers were treated with Ris or EuE-Ris hydrogels. All the lesions that received topical Ris or EuE-Ris showed an improvement with respect to control: reduction of ulcer average size, cicatrization, flattened edges and no signs of necrosis. In addition, a marked parasitic inhibition of 69.5 and 73.7% was observed in the groups treated with Ris and EuE-Ris, respectively, with the IgG2a levels indicating a tendency towards cure. The results are promising and the system should now be enhanced to achieve total parasite elimination. Elsevier 2021-05-29 /pmc/articles/PMC8173260/ /pubmed/34124403 http://dx.doi.org/10.1016/j.heliyon.2021.e07136 Text en © 2021 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Peralta, Ma. Florencia
Guzman, Ma. Laura
Bracamonte, Ma. Estefanía
Marco, J. Diego
Olivera, Ma. Eugenia
Carrer, Dolores C.
Barroso, Paola A.
Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title_full Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title_fullStr Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title_full_unstemmed Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title_short Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
title_sort efficacy of topical risedronate and risedronate - eudragit e complex in a model of cutaneous leishmaniasis induced by leishmania (leishmania) amazonensis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173260/
https://www.ncbi.nlm.nih.gov/pubmed/34124403
http://dx.doi.org/10.1016/j.heliyon.2021.e07136
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