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Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis
An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173260/ https://www.ncbi.nlm.nih.gov/pubmed/34124403 http://dx.doi.org/10.1016/j.heliyon.2021.e07136 |
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author | Peralta, Ma. Florencia Guzman, Ma. Laura Bracamonte, Ma. Estefanía Marco, J. Diego Olivera, Ma. Eugenia Carrer, Dolores C. Barroso, Paola A. |
author_facet | Peralta, Ma. Florencia Guzman, Ma. Laura Bracamonte, Ma. Estefanía Marco, J. Diego Olivera, Ma. Eugenia Carrer, Dolores C. Barroso, Paola A. |
author_sort | Peralta, Ma. Florencia |
collection | PubMed |
description | An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work was to investigate the in vivo efficacy of topical Ris and EuE-Ris complexes on CL. Surface charge and Ris release kinetics from the different dispersions were analyzed. BALB/c mice were infected intradermally with promastigotes of L. (L.) amazonensis. Ulcers were treated with Ris or EuE-Ris hydrogels. All the lesions that received topical Ris or EuE-Ris showed an improvement with respect to control: reduction of ulcer average size, cicatrization, flattened edges and no signs of necrosis. In addition, a marked parasitic inhibition of 69.5 and 73.7% was observed in the groups treated with Ris and EuE-Ris, respectively, with the IgG2a levels indicating a tendency towards cure. The results are promising and the system should now be enhanced to achieve total parasite elimination. |
format | Online Article Text |
id | pubmed-8173260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81732602021-06-11 Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis Peralta, Ma. Florencia Guzman, Ma. Laura Bracamonte, Ma. Estefanía Marco, J. Diego Olivera, Ma. Eugenia Carrer, Dolores C. Barroso, Paola A. Heliyon Research Article An efficacious topical treatment for cutaneous leishmaniasis (CL) is highly desirable but still an ongoing challenge. Systemic risedronate (Ris) has been reported to have anti-leishmanial properties and Eudragit EPO (EuE) has shown in vitro activity against L. (L.) amazonensis. The aim of this work was to investigate the in vivo efficacy of topical Ris and EuE-Ris complexes on CL. Surface charge and Ris release kinetics from the different dispersions were analyzed. BALB/c mice were infected intradermally with promastigotes of L. (L.) amazonensis. Ulcers were treated with Ris or EuE-Ris hydrogels. All the lesions that received topical Ris or EuE-Ris showed an improvement with respect to control: reduction of ulcer average size, cicatrization, flattened edges and no signs of necrosis. In addition, a marked parasitic inhibition of 69.5 and 73.7% was observed in the groups treated with Ris and EuE-Ris, respectively, with the IgG2a levels indicating a tendency towards cure. The results are promising and the system should now be enhanced to achieve total parasite elimination. Elsevier 2021-05-29 /pmc/articles/PMC8173260/ /pubmed/34124403 http://dx.doi.org/10.1016/j.heliyon.2021.e07136 Text en © 2021 Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Peralta, Ma. Florencia Guzman, Ma. Laura Bracamonte, Ma. Estefanía Marco, J. Diego Olivera, Ma. Eugenia Carrer, Dolores C. Barroso, Paola A. Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title | Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title_full | Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title_fullStr | Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title_full_unstemmed | Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title_short | Efficacy of topical risedronate and risedronate - Eudragit E complex in a model of cutaneous leishmaniasis induced by Leishmania (Leishmania) amazonensis |
title_sort | efficacy of topical risedronate and risedronate - eudragit e complex in a model of cutaneous leishmaniasis induced by leishmania (leishmania) amazonensis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173260/ https://www.ncbi.nlm.nih.gov/pubmed/34124403 http://dx.doi.org/10.1016/j.heliyon.2021.e07136 |
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