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FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis

BACKGROUND: As an important subfamily of arabinogalactan proteins (AGPs), fasciclin-like AGPs (FLAs) contribute to various aspects of growth, development and adaptation, yet their function remains largely elusive. Despite the diversity of FLAs, only two members, Arabidopsis FLA3 and rice MTR1, are r...

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Autores principales: Miao, Yingjing, Cao, Jiashu, Huang, Li, Yu, Youjian, Lin, Sue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173729/
https://www.ncbi.nlm.nih.gov/pubmed/34082704
http://dx.doi.org/10.1186/s12870-021-03038-x
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author Miao, Yingjing
Cao, Jiashu
Huang, Li
Yu, Youjian
Lin, Sue
author_facet Miao, Yingjing
Cao, Jiashu
Huang, Li
Yu, Youjian
Lin, Sue
author_sort Miao, Yingjing
collection PubMed
description BACKGROUND: As an important subfamily of arabinogalactan proteins (AGPs), fasciclin-like AGPs (FLAs) contribute to various aspects of growth, development and adaptation, yet their function remains largely elusive. Despite the diversity of FLAs, only two members, Arabidopsis FLA3 and rice MTR1, are reported to be involved in sexual reproduction. In this study, another Arabidopsis FLA-encoding gene, FLA14, was identified, and its role was investigated. RESULTS: Arabidopsis FLA14 was found to be a pollen grain-specific gene. Expression results from fusion with green fluorescent protein showed that FLA14 was localized along the cell membrane and in Hechtian strands. A loss-of-function mutant of FLA14 showed no discernible defects during male gametogenesis, but precocious pollen germination occurred inside the mature anthers under high moisture conditions. Overexpression of FLA14 caused 39.2% abnormal pollen grains with a shrunken and withered appearance, leading to largely reduced fertility with short mature siliques and lower seed set. Cytological and ultramicroscopic observation showed that ectopic expression of FLA14 caused disruption at the uninucleate stage, resulting in either collapsed pollen with absent intine or pollen of normal appearance but with a thickened intine. CONCLUSIONS: Taken together, our data suggest a role for FLA14 in pollen development and preventing premature pollen germination inside the anthers under high relative humidity in Arabidopsis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03038-x.
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spelling pubmed-81737292021-06-03 FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis Miao, Yingjing Cao, Jiashu Huang, Li Yu, Youjian Lin, Sue BMC Plant Biol Research BACKGROUND: As an important subfamily of arabinogalactan proteins (AGPs), fasciclin-like AGPs (FLAs) contribute to various aspects of growth, development and adaptation, yet their function remains largely elusive. Despite the diversity of FLAs, only two members, Arabidopsis FLA3 and rice MTR1, are reported to be involved in sexual reproduction. In this study, another Arabidopsis FLA-encoding gene, FLA14, was identified, and its role was investigated. RESULTS: Arabidopsis FLA14 was found to be a pollen grain-specific gene. Expression results from fusion with green fluorescent protein showed that FLA14 was localized along the cell membrane and in Hechtian strands. A loss-of-function mutant of FLA14 showed no discernible defects during male gametogenesis, but precocious pollen germination occurred inside the mature anthers under high moisture conditions. Overexpression of FLA14 caused 39.2% abnormal pollen grains with a shrunken and withered appearance, leading to largely reduced fertility with short mature siliques and lower seed set. Cytological and ultramicroscopic observation showed that ectopic expression of FLA14 caused disruption at the uninucleate stage, resulting in either collapsed pollen with absent intine or pollen of normal appearance but with a thickened intine. CONCLUSIONS: Taken together, our data suggest a role for FLA14 in pollen development and preventing premature pollen germination inside the anthers under high relative humidity in Arabidopsis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-021-03038-x. BioMed Central 2021-06-03 /pmc/articles/PMC8173729/ /pubmed/34082704 http://dx.doi.org/10.1186/s12870-021-03038-x Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Miao, Yingjing
Cao, Jiashu
Huang, Li
Yu, Youjian
Lin, Sue
FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title_full FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title_fullStr FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title_full_unstemmed FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title_short FLA14 is required for pollen development and preventing premature pollen germination under high humidity in Arabidopsis
title_sort fla14 is required for pollen development and preventing premature pollen germination under high humidity in arabidopsis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173729/
https://www.ncbi.nlm.nih.gov/pubmed/34082704
http://dx.doi.org/10.1186/s12870-021-03038-x
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