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Optimization of protocols for pre-embedding immunogold electron microscopy of neurons in cell cultures and brains

Immunogold labeling allows localization of proteins at the electron microscopy (EM) level of resolution, and quantification of signals. The present paper summarizes methodological issues and experiences gained from studies on the distribution of synaptic and other neuron-specific proteins in cell cu...

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Detalles Bibliográficos
Autores principales:	Tao-Cheng, Jung-Hwa, Crocker, Virginia, Moreira, Sandra Lara, Azzam, Rita
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2021
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173732/ https://www.ncbi.nlm.nih.gov/pubmed/34082785 http://dx.doi.org/10.1186/s13041-021-00799-2

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