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Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly

Proliferation of pancreatic β-cells has long been known to reach its peak in the neonatal stages and decline during adulthood. However, β-cell proliferation has been studied under the assumption that all β-cells constitute a single, homogenous population. It is unknown whether a subpopulation of β-c...

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Autores principales: Lee, Sharon, Zhang, Jing, Saravanakumar, Supraja, Flisher, Marcus F., Grimm, David R., van der Meulen, Talitha, Huising, Mark O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Diabetes Association 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173805/
https://www.ncbi.nlm.nih.gov/pubmed/33563657
http://dx.doi.org/10.2337/db20-0679
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author Lee, Sharon
Zhang, Jing
Saravanakumar, Supraja
Flisher, Marcus F.
Grimm, David R.
van der Meulen, Talitha
Huising, Mark O.
author_facet Lee, Sharon
Zhang, Jing
Saravanakumar, Supraja
Flisher, Marcus F.
Grimm, David R.
van der Meulen, Talitha
Huising, Mark O.
author_sort Lee, Sharon
collection PubMed
description Proliferation of pancreatic β-cells has long been known to reach its peak in the neonatal stages and decline during adulthood. However, β-cell proliferation has been studied under the assumption that all β-cells constitute a single, homogenous population. It is unknown whether a subpopulation of β-cells retains the capacity to proliferate at a higher rate and thus contributes disproportionately to the maintenance of mature β-cell mass in adults. We therefore assessed the proliferative capacity and turnover potential of virgin β-cells, a novel population of immature β-cells found at the islet periphery. We demonstrate that virgin β-cells can proliferate but do so at rates similar to those of mature β-cells from the same islet under normal and challenged conditions. Virgin β-cell proliferation rates also conform to the age-dependent decline previously reported for β-cells at large. We further show that virgin β-cells represent a long-lived, stable subpopulation of β-cells with low turnover into mature β-cells under healthy conditions. Our observations indicate that virgin β-cells at the islet periphery can divide but do not contribute disproportionately to the maintenance of adult β-cell mass.
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spelling pubmed-81738052022-05-01 Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly Lee, Sharon Zhang, Jing Saravanakumar, Supraja Flisher, Marcus F. Grimm, David R. van der Meulen, Talitha Huising, Mark O. Diabetes Islet Studies Proliferation of pancreatic β-cells has long been known to reach its peak in the neonatal stages and decline during adulthood. However, β-cell proliferation has been studied under the assumption that all β-cells constitute a single, homogenous population. It is unknown whether a subpopulation of β-cells retains the capacity to proliferate at a higher rate and thus contributes disproportionately to the maintenance of mature β-cell mass in adults. We therefore assessed the proliferative capacity and turnover potential of virgin β-cells, a novel population of immature β-cells found at the islet periphery. We demonstrate that virgin β-cells can proliferate but do so at rates similar to those of mature β-cells from the same islet under normal and challenged conditions. Virgin β-cell proliferation rates also conform to the age-dependent decline previously reported for β-cells at large. We further show that virgin β-cells represent a long-lived, stable subpopulation of β-cells with low turnover into mature β-cells under healthy conditions. Our observations indicate that virgin β-cells at the islet periphery can divide but do not contribute disproportionately to the maintenance of adult β-cell mass. American Diabetes Association 2021-05 2021-02-09 /pmc/articles/PMC8173805/ /pubmed/33563657 http://dx.doi.org/10.2337/db20-0679 Text en © 2021 by the American Diabetes Association https://www.diabetesjournals.org/content/licenseReaders may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at https://www.diabetesjournals.org/content/license.
spellingShingle Islet Studies
Lee, Sharon
Zhang, Jing
Saravanakumar, Supraja
Flisher, Marcus F.
Grimm, David R.
van der Meulen, Talitha
Huising, Mark O.
Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title_full Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title_fullStr Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title_full_unstemmed Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title_short Virgin β-Cells at the Neogenic Niche Proliferate Normally and Mature Slowly
title_sort virgin β-cells at the neogenic niche proliferate normally and mature slowly
topic Islet Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8173805/
https://www.ncbi.nlm.nih.gov/pubmed/33563657
http://dx.doi.org/10.2337/db20-0679
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