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CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia

Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting...

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Autores principales: Sampaio, Pedro, da Silva, Mónica Ferro, Vale, Inês, Roxo-Rosa, Mónica, Pinto, Andreia, Constant, Carolina, Pereira, Luisa, Quintão, Carla M., Lopes, Susana S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: European Respiratory Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174773/
https://www.ncbi.nlm.nih.gov/pubmed/34104642
http://dx.doi.org/10.1183/23120541.00792-2020
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author Sampaio, Pedro
da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
author_facet Sampaio, Pedro
da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
author_sort Sampaio, Pedro
collection PubMed
description Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers. Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients. The correlation coefficient between the two methods was R(2)=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18–9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30 Hz. We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases.
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spelling pubmed-81747732021-06-07 CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia Sampaio, Pedro da Silva, Mónica Ferro Vale, Inês Roxo-Rosa, Mónica Pinto, Andreia Constant, Carolina Pereira, Luisa Quintão, Carla M. Lopes, Susana S. ERJ Open Res Original Articles Evaluation of ciliary beat frequency (CBF) performed by high-speed videomicroscopy analysis (HVMA) is one of the techniques required for the correct diagnosis of primary ciliary dyskinesia (PCD). Currently, due to lack of open-source software, this technique is widely performed by visually counting the ciliary beatings per a given time-window. Our aim was to generate open-source, fast and intuitive software for evaluating CBF, validated in Portuguese PCD patients and healthy volunteers. Nasal brushings collected from 17 adult healthy volunteers and 34 PCD-referred subjects were recorded using HVMA. Evaluation of CBF was compared by two different methodologies: the new semi-automated computer software CiliarMove and the manual observation method using slow-motion movies. Clinical history, nasal nitric oxide and transmission electron microscopy were performed for diagnosis of PCD in the patient group. Genetic analysis was performed in a subset (n=8) of suspected PCD patients. The correlation coefficient between the two methods was R(2)=0.9895. The interval of CBF values obtained from the healthy control group (n=17) was 6.18–9.17 Hz at 25°C. In the PCD-excluded group (n=16), CBF ranged from 6.84 to 10.93 Hz and in the PCD group (n=18), CBF ranged from 0 to 14.30 Hz. We offer an automated open-source programme named CiliarMove, validated by the manual observation method in a healthy volunteer control group, a PCD-excluded group and a PCD-confirmed group. In our hands, comparisons between CBF intervals alone could discern between healthy and PCD groups in 78% of the cases. European Respiratory Society 2021-02-08 /pmc/articles/PMC8174773/ /pubmed/34104642 http://dx.doi.org/10.1183/23120541.00792-2020 Text en Copyright ©ERS 2021 https://creativecommons.org/licenses/by-nc/4.0/This article is open access and distributed under the terms of the Creative Commons Attribution Non-Commercial Licence 4.0.
spellingShingle Original Articles
Sampaio, Pedro
da Silva, Mónica Ferro
Vale, Inês
Roxo-Rosa, Mónica
Pinto, Andreia
Constant, Carolina
Pereira, Luisa
Quintão, Carla M.
Lopes, Susana S.
CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title_full CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title_fullStr CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title_full_unstemmed CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title_short CiliarMove: new software for evaluating ciliary beat frequency helps find novel mutations by a Portuguese multidisciplinary team on primary ciliary dyskinesia
title_sort ciliarmove: new software for evaluating ciliary beat frequency helps find novel mutations by a portuguese multidisciplinary team on primary ciliary dyskinesia
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174773/
https://www.ncbi.nlm.nih.gov/pubmed/34104642
http://dx.doi.org/10.1183/23120541.00792-2020
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