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Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS
Huashi Baidu prescription (HSBDF), recommended in the Guideline for the Diagnosis and Treatment of Novel Coronavirus (2019-nCoV) Pneumonia (On Trials, the Seventh Edition), was clinically used to treat severe corona virus disease 2019 (COVID-19) with cough, blood-stained sputum, inhibited defecation...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
China Pharmaceutical University. Published by Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174777/ https://www.ncbi.nlm.nih.gov/pubmed/34092298 http://dx.doi.org/10.1016/S1875-5364(21)60046-8 |
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author | WEI, Wen-Long WU, Shi-Fei LI, Hao-Jv LI, Zhen-Wei QU, Hua YAO, Chang-Liang ZHANG, Jian-Qing LI, Jia-Yuan WU, Wan-Ying GUO, De-An |
author_facet | WEI, Wen-Long WU, Shi-Fei LI, Hao-Jv LI, Zhen-Wei QU, Hua YAO, Chang-Liang ZHANG, Jian-Qing LI, Jia-Yuan WU, Wan-Ying GUO, De-An |
author_sort | WEI, Wen-Long |
collection | PubMed |
description | Huashi Baidu prescription (HSBDF), recommended in the Guideline for the Diagnosis and Treatment of Novel Coronavirus (2019-nCoV) Pneumonia (On Trials, the Seventh Edition), was clinically used to treat severe corona virus disease 2019 (COVID-19) with cough, blood-stained sputum, inhibited defecation, red tongue etc. symptoms. This study was aimed to elucidate and profile the knowledge on its chemical constituents and the potential anti-inflammatory effect in vitro. In the study, the chemical constituents in extract of HSBDF were characterized by UPLC-Q-TOF/MS in both negative and positive modes, and the pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays (ELISA) to determine the effects of HSBDF in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that a total of 217 chemical constituents were tentativedly characterized in HSBDF. Moreover, HSBDF could alleviate the expression levels of IL-6 and TNF-α in the cell models, indicating that the antiviral effects of HSBDF might be associated with regulation of the inflammatory cytokines production in RAW264.7 cells. We hope that the results could be served as the basic data for further study of HSBDF on anti-COVID-19 effect. |
format | Online Article Text |
id | pubmed-8174777 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | China Pharmaceutical University. Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81747772021-06-04 Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS WEI, Wen-Long WU, Shi-Fei LI, Hao-Jv LI, Zhen-Wei QU, Hua YAO, Chang-Liang ZHANG, Jian-Qing LI, Jia-Yuan WU, Wan-Ying GUO, De-An Chin J Nat Med Research Article Huashi Baidu prescription (HSBDF), recommended in the Guideline for the Diagnosis and Treatment of Novel Coronavirus (2019-nCoV) Pneumonia (On Trials, the Seventh Edition), was clinically used to treat severe corona virus disease 2019 (COVID-19) with cough, blood-stained sputum, inhibited defecation, red tongue etc. symptoms. This study was aimed to elucidate and profile the knowledge on its chemical constituents and the potential anti-inflammatory effect in vitro. In the study, the chemical constituents in extract of HSBDF were characterized by UPLC-Q-TOF/MS in both negative and positive modes, and the pro-inflammatory cytokines were measured by enzyme-linked immunosorbent assays (ELISA) to determine the effects of HSBDF in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The results showed that a total of 217 chemical constituents were tentativedly characterized in HSBDF. Moreover, HSBDF could alleviate the expression levels of IL-6 and TNF-α in the cell models, indicating that the antiviral effects of HSBDF might be associated with regulation of the inflammatory cytokines production in RAW264.7 cells. We hope that the results could be served as the basic data for further study of HSBDF on anti-COVID-19 effect. China Pharmaceutical University. Published by Elsevier B.V. 2021-06 2021-06-03 /pmc/articles/PMC8174777/ /pubmed/34092298 http://dx.doi.org/10.1016/S1875-5364(21)60046-8 Text en © 2021 China Pharmaceutical University Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Research Article WEI, Wen-Long WU, Shi-Fei LI, Hao-Jv LI, Zhen-Wei QU, Hua YAO, Chang-Liang ZHANG, Jian-Qing LI, Jia-Yuan WU, Wan-Ying GUO, De-An Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title | Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title_full | Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title_fullStr | Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title_full_unstemmed | Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title_short | Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS |
title_sort | chemical profiling of huashi baidu prescription, an effective anti-covid-19 tcm formula, by uplc-q-tof/ms |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8174777/ https://www.ncbi.nlm.nih.gov/pubmed/34092298 http://dx.doi.org/10.1016/S1875-5364(21)60046-8 |
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