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SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia
COVID-19 pandemic severely impacted the healthcare and economy on a global scale. It is widely recognized that mass testing is an efficient way to contain the spread of SARS-CoV-2 infection as well as aid in the development of informed policies for disease management. However, the current COVID-19 w...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Published by Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175123/ https://www.ncbi.nlm.nih.gov/pubmed/34091213 http://dx.doi.org/10.1016/j.jviromet.2021.114199 |
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author | Avetyan, Diana Chavushyan, Andranik Ghazaryan, Hovsep Melkonyan, Ani Stepanyan, Ani Zakharyan, Roksana Hayrapetyan, Varduhi Atshemyan, Sofi Khachatryan, Gisane Sirunyan, Tamara Davitavyan, Suren Martirosyan, Gevorg Melik-Andreasyan, Gayane Sargsyan, Shushan Ghazazyan, Armine Aleksanyan, Naira Yin, Xiushan Arakelyan, Arsen |
author_facet | Avetyan, Diana Chavushyan, Andranik Ghazaryan, Hovsep Melkonyan, Ani Stepanyan, Ani Zakharyan, Roksana Hayrapetyan, Varduhi Atshemyan, Sofi Khachatryan, Gisane Sirunyan, Tamara Davitavyan, Suren Martirosyan, Gevorg Melik-Andreasyan, Gayane Sargsyan, Shushan Ghazazyan, Armine Aleksanyan, Naira Yin, Xiushan Arakelyan, Arsen |
author_sort | Avetyan, Diana |
collection | PubMed |
description | COVID-19 pandemic severely impacted the healthcare and economy on a global scale. It is widely recognized that mass testing is an efficient way to contain the spread of SARS-CoV-2 infection as well as aid in the development of informed policies for disease management. However, the current COVID-19 worldwide infection rates increased the demand for rapid and reliable screening of infection. We compared the performance of qRT-PCR in direct heat-inactivated (H), heat-inactivated and pelleted (HC) samples against RNA in a group of 74 subjects (44 positive and 30 negative). Then we compared the sensitivity of HC in a larger group of 196 COVID-19 positive samples. Our study suggests that HC samples show higher accuracy for SARS-CoV-2 detection PCR assay compared to direct H (89 % vs 83 % of the detection in RNA). The sensitivity of detection using direct samples varied depending on the sample transport and storage media as well as the viral loads (as measured by qRT-PCR Ct levels). Altogether, all the data suggest that purified RNA provides more accurate results, however, direct sample testing with qRT-PCR may help to significantly increase testing capacity. Switching to the direct sample testing is justified if the number of tests is doubled at least. |
format | Online Article Text |
id | pubmed-8175123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Published by Elsevier B.V. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81751232021-06-04 SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia Avetyan, Diana Chavushyan, Andranik Ghazaryan, Hovsep Melkonyan, Ani Stepanyan, Ani Zakharyan, Roksana Hayrapetyan, Varduhi Atshemyan, Sofi Khachatryan, Gisane Sirunyan, Tamara Davitavyan, Suren Martirosyan, Gevorg Melik-Andreasyan, Gayane Sargsyan, Shushan Ghazazyan, Armine Aleksanyan, Naira Yin, Xiushan Arakelyan, Arsen J Virol Methods Article COVID-19 pandemic severely impacted the healthcare and economy on a global scale. It is widely recognized that mass testing is an efficient way to contain the spread of SARS-CoV-2 infection as well as aid in the development of informed policies for disease management. However, the current COVID-19 worldwide infection rates increased the demand for rapid and reliable screening of infection. We compared the performance of qRT-PCR in direct heat-inactivated (H), heat-inactivated and pelleted (HC) samples against RNA in a group of 74 subjects (44 positive and 30 negative). Then we compared the sensitivity of HC in a larger group of 196 COVID-19 positive samples. Our study suggests that HC samples show higher accuracy for SARS-CoV-2 detection PCR assay compared to direct H (89 % vs 83 % of the detection in RNA). The sensitivity of detection using direct samples varied depending on the sample transport and storage media as well as the viral loads (as measured by qRT-PCR Ct levels). Altogether, all the data suggest that purified RNA provides more accurate results, however, direct sample testing with qRT-PCR may help to significantly increase testing capacity. Switching to the direct sample testing is justified if the number of tests is doubled at least. Published by Elsevier B.V. 2021-09 2021-06-04 /pmc/articles/PMC8175123/ /pubmed/34091213 http://dx.doi.org/10.1016/j.jviromet.2021.114199 Text en © 2021 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Avetyan, Diana Chavushyan, Andranik Ghazaryan, Hovsep Melkonyan, Ani Stepanyan, Ani Zakharyan, Roksana Hayrapetyan, Varduhi Atshemyan, Sofi Khachatryan, Gisane Sirunyan, Tamara Davitavyan, Suren Martirosyan, Gevorg Melik-Andreasyan, Gayane Sargsyan, Shushan Ghazazyan, Armine Aleksanyan, Naira Yin, Xiushan Arakelyan, Arsen SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title_full | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title_fullStr | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title_full_unstemmed | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title_short | SARS-CoV-2 detection by extraction-free qRT-PCR for massive and rapid COVID-19 diagnosis during a pandemic in Armenia |
title_sort | sars-cov-2 detection by extraction-free qrt-pcr for massive and rapid covid-19 diagnosis during a pandemic in armenia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175123/ https://www.ncbi.nlm.nih.gov/pubmed/34091213 http://dx.doi.org/10.1016/j.jviromet.2021.114199 |
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