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Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats

Aging affects the brain function in elderly individuals, and Dushen Tang (DST) is widely used for the treatment of senile diseases. In this study, the protective effect of DST against memory impairment was evaluated through the Morris water maze (MWM) test and transmission electron microscopy (TEM)....

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Autores principales: Wang, Jifeng, He, Min, Guo, Wenjun, Zhang, Yanhong, Sui, Xin, Lin, Jianan, Liu, Xiaoran, Li, Hui, Li, Jing, Yang, Qing, Kan, Mo, Zhang, Zhuang, Ming, Sitong, Qu, Xiaobo, Li, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175156/
https://www.ncbi.nlm.nih.gov/pubmed/34136571
http://dx.doi.org/10.1155/2021/6649085
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author Wang, Jifeng
He, Min
Guo, Wenjun
Zhang, Yanhong
Sui, Xin
Lin, Jianan
Liu, Xiaoran
Li, Hui
Li, Jing
Yang, Qing
Kan, Mo
Zhang, Zhuang
Ming, Sitong
Qu, Xiaobo
Li, Na
author_facet Wang, Jifeng
He, Min
Guo, Wenjun
Zhang, Yanhong
Sui, Xin
Lin, Jianan
Liu, Xiaoran
Li, Hui
Li, Jing
Yang, Qing
Kan, Mo
Zhang, Zhuang
Ming, Sitong
Qu, Xiaobo
Li, Na
author_sort Wang, Jifeng
collection PubMed
description Aging affects the brain function in elderly individuals, and Dushen Tang (DST) is widely used for the treatment of senile diseases. In this study, the protective effect of DST against memory impairment was evaluated through the Morris water maze (MWM) test and transmission electron microscopy (TEM). A joint analysis was also performed using LC-MS metabolomics and the microbiome. The MWM test showed that DST could significantly improve the spatial memory and learning abilities of rats with memory impairment, and the TEM analysis showed that DST could reduce neuronal damage in the hippocampus of rats with memory impairment. Ten potential biomarkers involving pyruvate metabolism, the synthesis and degradation of ketone bodies, and other metabolic pathways were identified by the metabolomic analysis, and it was found that 3-hydroxybutyric acid and lactic acid were involved in the activation of cAMP signaling pathways. The 16S rDNA sequencing results showed that DST could regulate the structure of the gut microbiota in rats with memory impairment, and these effects were manifested as changes in energy metabolism. These findings suggest that DST exerts a good therapeutic effect on rats with memory impairment and that this effect might be mainly achieved by improving energy metabolism. These findings might lead to the potential development of DST as a drug for the treatment of rats with memory impairment.
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spelling pubmed-81751562021-06-15 Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats Wang, Jifeng He, Min Guo, Wenjun Zhang, Yanhong Sui, Xin Lin, Jianan Liu, Xiaoran Li, Hui Li, Jing Yang, Qing Kan, Mo Zhang, Zhuang Ming, Sitong Qu, Xiaobo Li, Na Biomed Res Int Research Article Aging affects the brain function in elderly individuals, and Dushen Tang (DST) is widely used for the treatment of senile diseases. In this study, the protective effect of DST against memory impairment was evaluated through the Morris water maze (MWM) test and transmission electron microscopy (TEM). A joint analysis was also performed using LC-MS metabolomics and the microbiome. The MWM test showed that DST could significantly improve the spatial memory and learning abilities of rats with memory impairment, and the TEM analysis showed that DST could reduce neuronal damage in the hippocampus of rats with memory impairment. Ten potential biomarkers involving pyruvate metabolism, the synthesis and degradation of ketone bodies, and other metabolic pathways were identified by the metabolomic analysis, and it was found that 3-hydroxybutyric acid and lactic acid were involved in the activation of cAMP signaling pathways. The 16S rDNA sequencing results showed that DST could regulate the structure of the gut microbiota in rats with memory impairment, and these effects were manifested as changes in energy metabolism. These findings suggest that DST exerts a good therapeutic effect on rats with memory impairment and that this effect might be mainly achieved by improving energy metabolism. These findings might lead to the potential development of DST as a drug for the treatment of rats with memory impairment. Hindawi 2021-05-27 /pmc/articles/PMC8175156/ /pubmed/34136571 http://dx.doi.org/10.1155/2021/6649085 Text en Copyright © 2021 Jifeng Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Jifeng
He, Min
Guo, Wenjun
Zhang, Yanhong
Sui, Xin
Lin, Jianan
Liu, Xiaoran
Li, Hui
Li, Jing
Yang, Qing
Kan, Mo
Zhang, Zhuang
Ming, Sitong
Qu, Xiaobo
Li, Na
Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title_full Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title_fullStr Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title_full_unstemmed Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title_short Microbiome-Metabolomics Reveals Endogenous Alterations of Energy Metabolism by the Dushen Tang to Attenuate D-Galactose-Induced Memory Impairment in Rats
title_sort microbiome-metabolomics reveals endogenous alterations of energy metabolism by the dushen tang to attenuate d-galactose-induced memory impairment in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175156/
https://www.ncbi.nlm.nih.gov/pubmed/34136571
http://dx.doi.org/10.1155/2021/6649085
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