Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution

Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in hum...

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Autores principales: Colombo, Federico, Corsiero, Elisa, Lewis, Myles J., Pitzalis, Costantino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175367/
https://www.ncbi.nlm.nih.gov/pubmed/34083682
http://dx.doi.org/10.1038/s41598-021-91264-6
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author Colombo, Federico
Corsiero, Elisa
Lewis, Myles J.
Pitzalis, Costantino
author_facet Colombo, Federico
Corsiero, Elisa
Lewis, Myles J.
Pitzalis, Costantino
author_sort Colombo, Federico
collection PubMed
description Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution.
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spelling pubmed-81753672021-06-04 Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution Colombo, Federico Corsiero, Elisa Lewis, Myles J. Pitzalis, Costantino Sci Rep Article Since the first report of SARS-CoV-2 in China in 2019, there has been a huge debate about the origin. In this work, using a different method we aimed to strengthen the observation that no evidence of genetic manipulation has been found by (1) detecting classical restriction site (RS) sequence in human SARS-CoV-2 genomes and (2) comparing them with other recombinant SARS-CoV-like virus created for experimental purposes. Finally, we propose a novel approach consisting in the generation of a restriction endonucleases site map of SARS-CoV-2 and other related coronavirus genomes to be used as a fingerprint to trace the virus evolution. Nature Publishing Group UK 2021-06-03 /pmc/articles/PMC8175367/ /pubmed/34083682 http://dx.doi.org/10.1038/s41598-021-91264-6 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Colombo, Federico
Corsiero, Elisa
Lewis, Myles J.
Pitzalis, Costantino
Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_full Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_fullStr Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_full_unstemmed Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_short Generation of restriction endonucleases barcode map to trace SARS-CoV-2 origin and evolution
title_sort generation of restriction endonucleases barcode map to trace sars-cov-2 origin and evolution
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175367/
https://www.ncbi.nlm.nih.gov/pubmed/34083682
http://dx.doi.org/10.1038/s41598-021-91264-6
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