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Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens

ABSTRACT: Attenuated Listeria monocytogenes could be a potential vaccine vector for the immunotherapy of tumors or pathogens. However, the lack of reliable promoters has limited its ability to express foreign antigens. In the present study, 21 promoters were identified from Listeria monocytogenes th...

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Autores principales: Ma, Junfei, Ji, Qianyu, Wang, Shuying, Qiu, Jingxuan, Liu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175932/
https://www.ncbi.nlm.nih.gov/pubmed/34086117
http://dx.doi.org/10.1007/s00253-021-11374-z
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author Ma, Junfei
Ji, Qianyu
Wang, Shuying
Qiu, Jingxuan
Liu, Qing
author_facet Ma, Junfei
Ji, Qianyu
Wang, Shuying
Qiu, Jingxuan
Liu, Qing
author_sort Ma, Junfei
collection PubMed
description ABSTRACT: Attenuated Listeria monocytogenes could be a potential vaccine vector for the immunotherapy of tumors or pathogens. However, the lack of reliable promoters has limited its ability to express foreign antigens. In the present study, 21 promoters were identified from Listeria monocytogenes through RNA-seq analysis under two pH conditions of pH 7.4 and pH 5.5. Based on the constructed fluorescence report system, 7 constitutive promoters exhibited higher strength than P(help) (1.8-fold to 5.4-fold), a previously reported strong promoter. Furthermore, the selected 5 constitutive promoters exhibited higher UreB production activity than P(help) (1.1-fold to 8.3-fold). Notably, a well-characterized constitutive promoter P(18) was found with the highest activity of fluorescence intensity and UreB production. In summary, the study provides a panel of strong constitutive promoters for Listeria monocytogenes and offers a theoretical basis for mining constitutive promoters in other organisms. KEY POINTS: • Twenty-one promoters were identified from L. monocytogenes through RNA-seq. • Fluorescent tracer of L. monocytogenes (P(18)) was performed in vitro and in vivo. • A well-characterized constitutive promoter P(18) could improve the expression level of a foreign antigen UreB in L. monocytogenes SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-021-11374-z.
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spelling pubmed-81759322021-06-04 Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens Ma, Junfei Ji, Qianyu Wang, Shuying Qiu, Jingxuan Liu, Qing Appl Microbiol Biotechnol Applied Microbial and Cell Physiology ABSTRACT: Attenuated Listeria monocytogenes could be a potential vaccine vector for the immunotherapy of tumors or pathogens. However, the lack of reliable promoters has limited its ability to express foreign antigens. In the present study, 21 promoters were identified from Listeria monocytogenes through RNA-seq analysis under two pH conditions of pH 7.4 and pH 5.5. Based on the constructed fluorescence report system, 7 constitutive promoters exhibited higher strength than P(help) (1.8-fold to 5.4-fold), a previously reported strong promoter. Furthermore, the selected 5 constitutive promoters exhibited higher UreB production activity than P(help) (1.1-fold to 8.3-fold). Notably, a well-characterized constitutive promoter P(18) was found with the highest activity of fluorescence intensity and UreB production. In summary, the study provides a panel of strong constitutive promoters for Listeria monocytogenes and offers a theoretical basis for mining constitutive promoters in other organisms. KEY POINTS: • Twenty-one promoters were identified from L. monocytogenes through RNA-seq. • Fluorescent tracer of L. monocytogenes (P(18)) was performed in vitro and in vivo. • A well-characterized constitutive promoter P(18) could improve the expression level of a foreign antigen UreB in L. monocytogenes SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00253-021-11374-z. Springer Berlin Heidelberg 2021-06-04 2021 /pmc/articles/PMC8175932/ /pubmed/34086117 http://dx.doi.org/10.1007/s00253-021-11374-z Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Applied Microbial and Cell Physiology
Ma, Junfei
Ji, Qianyu
Wang, Shuying
Qiu, Jingxuan
Liu, Qing
Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title_full Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title_fullStr Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title_full_unstemmed Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title_short Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens
title_sort identification and evaluation of a panel of strong constitutive promoters in listeria monocytogenes for improving the expression of foreign antigens
topic Applied Microbial and Cell Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8175932/
https://www.ncbi.nlm.nih.gov/pubmed/34086117
http://dx.doi.org/10.1007/s00253-021-11374-z
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