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Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites
In the current study, twenty-eight soil samples were collected from coalmine sites of Telangana, India. The isolates were purified and identified based on their culture characterization on oatmeal agar, glycerol asparagine agar, yeast extract-malt extract agar, inorganic salt starch agar, and starch...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176045/ https://www.ncbi.nlm.nih.gov/pubmed/34121898 http://dx.doi.org/10.1016/j.sjbs.2021.03.029 |
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author | Sarika, Kasarla Sampath, Gattu Kaveriyappan Govindarajan, Rasiravathanahalli Ameen, Fuad Alwakeel, Suaad Al Gwaiz, Hussah I. Raja Komuraiah, Thampu Ravi, Gangalla |
author_facet | Sarika, Kasarla Sampath, Gattu Kaveriyappan Govindarajan, Rasiravathanahalli Ameen, Fuad Alwakeel, Suaad Al Gwaiz, Hussah I. Raja Komuraiah, Thampu Ravi, Gangalla |
author_sort | Sarika, Kasarla |
collection | PubMed |
description | In the current study, twenty-eight soil samples were collected from coalmine sites of Telangana, India. The isolates were purified and identified based on their culture characterization on oatmeal agar, glycerol asparagine agar, yeast extract-malt extract agar, inorganic salt starch agar, and starch casein agar medium. Further, the supernatant of all the isolates were tested for antimicrobial and antifungal activities. The biochemical and microscopic studies of isolated strains results indicates the potential isolate strains belongs to Streptomyces genus. Among all the strains the biological activity of BHPL-KSKU5 showed higher anti-bacterial and anti-funagal activity. The molecular characterization of BHPL-KSKU5 16s rDNA gene sequence and phylogenetic tree showed that is mostly related to the Streptomysis felleus (S. felleus) strain. This isolate was submitted to gene bank NCBI with accession number MH553077. In addition, physiological studies such as utilization of carbon, nitrogen, amino acid sources of potential isolated were studied. Further, optimization, purification and characterization of the novel compound producing strain may be helpful for discovering the new therapeutic microbial agent. |
format | Online Article Text |
id | pubmed-8176045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81760452021-06-11 Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites Sarika, Kasarla Sampath, Gattu Kaveriyappan Govindarajan, Rasiravathanahalli Ameen, Fuad Alwakeel, Suaad Al Gwaiz, Hussah I. Raja Komuraiah, Thampu Ravi, Gangalla Saudi J Biol Sci Original Article In the current study, twenty-eight soil samples were collected from coalmine sites of Telangana, India. The isolates were purified and identified based on their culture characterization on oatmeal agar, glycerol asparagine agar, yeast extract-malt extract agar, inorganic salt starch agar, and starch casein agar medium. Further, the supernatant of all the isolates were tested for antimicrobial and antifungal activities. The biochemical and microscopic studies of isolated strains results indicates the potential isolate strains belongs to Streptomyces genus. Among all the strains the biological activity of BHPL-KSKU5 showed higher anti-bacterial and anti-funagal activity. The molecular characterization of BHPL-KSKU5 16s rDNA gene sequence and phylogenetic tree showed that is mostly related to the Streptomysis felleus (S. felleus) strain. This isolate was submitted to gene bank NCBI with accession number MH553077. In addition, physiological studies such as utilization of carbon, nitrogen, amino acid sources of potential isolated were studied. Further, optimization, purification and characterization of the novel compound producing strain may be helpful for discovering the new therapeutic microbial agent. Elsevier 2021-06 2021-03-17 /pmc/articles/PMC8176045/ /pubmed/34121898 http://dx.doi.org/10.1016/j.sjbs.2021.03.029 Text en © 2021 The Authors. Published by Elsevier B.V. on behalf of King Saud University. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Original Article Sarika, Kasarla Sampath, Gattu Kaveriyappan Govindarajan, Rasiravathanahalli Ameen, Fuad Alwakeel, Suaad Al Gwaiz, Hussah I. Raja Komuraiah, Thampu Ravi, Gangalla Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title | Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title_full | Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title_fullStr | Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title_full_unstemmed | Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title_short | Antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
title_sort | antimicrobial and antifungal activity of soil actinomycetes isolated from coal mine sites |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176045/ https://www.ncbi.nlm.nih.gov/pubmed/34121898 http://dx.doi.org/10.1016/j.sjbs.2021.03.029 |
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