Cargando…

Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications

After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remod...

Descripción completa

Detalles Bibliográficos
Autores principales: Marconi, Guya D., Fonticoli, Luigia, Rajan, Thangavelu Soundara, Lanuti, Paola, Della Rocca, Ylenia, Pierdomenico, Sante D., Trubiani, Oriana, Pizzicannella, Jacopo, Diomede, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176099/
https://www.ncbi.nlm.nih.gov/pubmed/34093237
http://dx.doi.org/10.3389/fphys.2021.676512
_version_ 1783703189217869824
author Marconi, Guya D.
Fonticoli, Luigia
Rajan, Thangavelu Soundara
Lanuti, Paola
Della Rocca, Ylenia
Pierdomenico, Sante D.
Trubiani, Oriana
Pizzicannella, Jacopo
Diomede, Francesca
author_facet Marconi, Guya D.
Fonticoli, Luigia
Rajan, Thangavelu Soundara
Lanuti, Paola
Della Rocca, Ylenia
Pierdomenico, Sante D.
Trubiani, Oriana
Pizzicannella, Jacopo
Diomede, Francesca
author_sort Marconi, Guya D.
collection PubMed
description After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-β1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (α-SMA), Vimentin, E-cadherin, β-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-β1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-β1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-β1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of α-SMA, Vimentin, E-cadherin, β-catenin, and Smad 2/3.
format Online
Article
Text
id pubmed-8176099
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-81760992021-06-05 Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications Marconi, Guya D. Fonticoli, Luigia Rajan, Thangavelu Soundara Lanuti, Paola Della Rocca, Ylenia Pierdomenico, Sante D. Trubiani, Oriana Pizzicannella, Jacopo Diomede, Francesca Front Physiol Physiology After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-β1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (α-SMA), Vimentin, E-cadherin, β-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-β1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-β1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-β1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of α-SMA, Vimentin, E-cadherin, β-catenin, and Smad 2/3. Frontiers Media S.A. 2021-05-21 /pmc/articles/PMC8176099/ /pubmed/34093237 http://dx.doi.org/10.3389/fphys.2021.676512 Text en Copyright © 2021 Marconi, Fonticoli, Rajan, Lanuti, Della Rocca, Pierdomenico, Trubiani, Pizzicannella and Diomede. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Marconi, Guya D.
Fonticoli, Luigia
Rajan, Thangavelu Soundara
Lanuti, Paola
Della Rocca, Ylenia
Pierdomenico, Sante D.
Trubiani, Oriana
Pizzicannella, Jacopo
Diomede, Francesca
Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title_full Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title_fullStr Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title_full_unstemmed Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title_short Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
title_sort transforming growth factor-beta1 and human gingival fibroblast-to-myofibroblast differentiation: molecular and morphological modifications
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176099/
https://www.ncbi.nlm.nih.gov/pubmed/34093237
http://dx.doi.org/10.3389/fphys.2021.676512
work_keys_str_mv AT marconiguyad transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT fonticoliluigia transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT rajanthangavelusoundara transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT lanutipaola transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT dellaroccaylenia transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT pierdomenicosanted transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT trubianioriana transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT pizzicannellajacopo transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications
AT diomedefrancesca transforminggrowthfactorbeta1andhumangingivalfibroblasttomyofibroblastdifferentiationmolecularandmorphologicalmodifications