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Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications
After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remod...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176099/ https://www.ncbi.nlm.nih.gov/pubmed/34093237 http://dx.doi.org/10.3389/fphys.2021.676512 |
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author | Marconi, Guya D. Fonticoli, Luigia Rajan, Thangavelu Soundara Lanuti, Paola Della Rocca, Ylenia Pierdomenico, Sante D. Trubiani, Oriana Pizzicannella, Jacopo Diomede, Francesca |
author_facet | Marconi, Guya D. Fonticoli, Luigia Rajan, Thangavelu Soundara Lanuti, Paola Della Rocca, Ylenia Pierdomenico, Sante D. Trubiani, Oriana Pizzicannella, Jacopo Diomede, Francesca |
author_sort | Marconi, Guya D. |
collection | PubMed |
description | After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-β1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (α-SMA), Vimentin, E-cadherin, β-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-β1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-β1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-β1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of α-SMA, Vimentin, E-cadherin, β-catenin, and Smad 2/3. |
format | Online Article Text |
id | pubmed-8176099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81760992021-06-05 Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications Marconi, Guya D. Fonticoli, Luigia Rajan, Thangavelu Soundara Lanuti, Paola Della Rocca, Ylenia Pierdomenico, Sante D. Trubiani, Oriana Pizzicannella, Jacopo Diomede, Francesca Front Physiol Physiology After oral mucosal injury, the healing response following specific steps that lead to wound closure and to tissue repair. Multiple cell populations are involved in this process; in particular, fibroblasts play a key role in the production of extracellular matrix (ECM). During wound healing the remodeling of ECM is a key stage to restore the tissue functionality through multifunctional fibroblast populations that are placed in the connective tissues of gingiva and periodontal ligament. Notably, a fibroblast sub-type (myofibroblast) is centrally involved in collagen synthesis and fibrillar remodeling. The present work evidenced the role of Transforming Growth Factor-beta1 (TGF-β1) to mediate human gingival fibroblasts (hGFs) differentiation into myofibroblasts derived from gingival fibroblasts (myo-hGFs). The morphological and functional features were analyzed through Confocal Laser Scanning Microscopy (CLSM), flow cytometry, and western blotting analyses. The specific markers, such as alpha-Smooth Muscle Actin (α-SMA), Vimentin, E-cadherin, β-catenin, and Smad 2/3, were modulated in myo-hGFs after the induction with TGF-β1, at different time points (24, 48, and 72 h). After 72 h of treatment TGF-β1 operates as an inducer of hGFs into myo-hGFs differentiation. We propose that TGF-β1 may promote in vitro the fibroblasts-to-myofibroblasts transition via the morphological and molecular modifications, as the induction of α-SMA, Vimentin, E-cadherin, β-catenin, and Smad 2/3. Frontiers Media S.A. 2021-05-21 /pmc/articles/PMC8176099/ /pubmed/34093237 http://dx.doi.org/10.3389/fphys.2021.676512 Text en Copyright © 2021 Marconi, Fonticoli, Rajan, Lanuti, Della Rocca, Pierdomenico, Trubiani, Pizzicannella and Diomede. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Marconi, Guya D. Fonticoli, Luigia Rajan, Thangavelu Soundara Lanuti, Paola Della Rocca, Ylenia Pierdomenico, Sante D. Trubiani, Oriana Pizzicannella, Jacopo Diomede, Francesca Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title | Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title_full | Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title_fullStr | Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title_full_unstemmed | Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title_short | Transforming Growth Factor-Beta1 and Human Gingival Fibroblast-to-Myofibroblast Differentiation: Molecular and Morphological Modifications |
title_sort | transforming growth factor-beta1 and human gingival fibroblast-to-myofibroblast differentiation: molecular and morphological modifications |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176099/ https://www.ncbi.nlm.nih.gov/pubmed/34093237 http://dx.doi.org/10.3389/fphys.2021.676512 |
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