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An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota

Some oligosaccharides induce growth of anti-inflammatory bacterial species and induce regulatory immunity in humans as well as animals. We have shown that the equine gut microbiota and the immune-microbial homeostasis largely stabilize within the first 50 days of life. Furthermore, we have previousl...

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Autores principales: Lindenberg, Frederikke Christine, Lützhøft, Ditte Olsen, Krych, Lukasz, Fielden, James, Kot, Witold, Frøkiær, Hanne, van Galen, Gaby, Nielsen, Dennis Sandris, Hansen, Axel Kornerup
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176217/
https://www.ncbi.nlm.nih.gov/pubmed/34093482
http://dx.doi.org/10.3389/fmicb.2021.666039
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author Lindenberg, Frederikke Christine
Lützhøft, Ditte Olsen
Krych, Lukasz
Fielden, James
Kot, Witold
Frøkiær, Hanne
van Galen, Gaby
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
author_facet Lindenberg, Frederikke Christine
Lützhøft, Ditte Olsen
Krych, Lukasz
Fielden, James
Kot, Witold
Frøkiær, Hanne
van Galen, Gaby
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
author_sort Lindenberg, Frederikke Christine
collection PubMed
description Some oligosaccharides induce growth of anti-inflammatory bacterial species and induce regulatory immunity in humans as well as animals. We have shown that the equine gut microbiota and the immune-microbial homeostasis largely stabilize within the first 50 days of life. Furthermore, we have previously established that certain bacterial species in the equine gut correlated with regulatory immunity. Accordingly, we hypothesized that an oligosaccharide rich diet fed to foals during the first 50 days would increase the abundance of bacterial species associated with regulatory immunity, and that this would influence immune responses in the foals. Eight pregnant mares and their foals were fed an oligosaccharide rich diet from 4 weeks before expected parturition until 49 days post-partum. Six mares and foals served as control. Fecal microbiota from mares and foals was characterized using 16S rRNA gene amplicon high throughput sequencing. On Day 49 the test foals had significantly higher abundances of Akkermansia spp. Blood sampled from the foals in the test group on Day 7, 28, and 49 showed non-significant increases in IgA, and decreases in IgG on Day 49. In BALB/cBomTac mice inoculated with gut microbiota from test and control foals we found increased species richness, increased relative abundance of several species identified as potentially anti-inflammatory in horses, which were unclassified Clostridiales, Ruminococcaceae, Ruminococcus, Oscilospira, and Coprococcus. We also found increased il10 expression in the ileum if inoculated with test foal microbiota. We conclude that an oligosaccharide diet fed to foals in the “window of opportunity,” the first 50 days of life, increases the abundance of anti-inflammatory species in the microbiota with potentially anti-inflammatory effects on regulatory immunity.
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spelling pubmed-81762172021-06-05 An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota Lindenberg, Frederikke Christine Lützhøft, Ditte Olsen Krych, Lukasz Fielden, James Kot, Witold Frøkiær, Hanne van Galen, Gaby Nielsen, Dennis Sandris Hansen, Axel Kornerup Front Microbiol Microbiology Some oligosaccharides induce growth of anti-inflammatory bacterial species and induce regulatory immunity in humans as well as animals. We have shown that the equine gut microbiota and the immune-microbial homeostasis largely stabilize within the first 50 days of life. Furthermore, we have previously established that certain bacterial species in the equine gut correlated with regulatory immunity. Accordingly, we hypothesized that an oligosaccharide rich diet fed to foals during the first 50 days would increase the abundance of bacterial species associated with regulatory immunity, and that this would influence immune responses in the foals. Eight pregnant mares and their foals were fed an oligosaccharide rich diet from 4 weeks before expected parturition until 49 days post-partum. Six mares and foals served as control. Fecal microbiota from mares and foals was characterized using 16S rRNA gene amplicon high throughput sequencing. On Day 49 the test foals had significantly higher abundances of Akkermansia spp. Blood sampled from the foals in the test group on Day 7, 28, and 49 showed non-significant increases in IgA, and decreases in IgG on Day 49. In BALB/cBomTac mice inoculated with gut microbiota from test and control foals we found increased species richness, increased relative abundance of several species identified as potentially anti-inflammatory in horses, which were unclassified Clostridiales, Ruminococcaceae, Ruminococcus, Oscilospira, and Coprococcus. We also found increased il10 expression in the ileum if inoculated with test foal microbiota. We conclude that an oligosaccharide diet fed to foals in the “window of opportunity,” the first 50 days of life, increases the abundance of anti-inflammatory species in the microbiota with potentially anti-inflammatory effects on regulatory immunity. Frontiers Media S.A. 2021-05-21 /pmc/articles/PMC8176217/ /pubmed/34093482 http://dx.doi.org/10.3389/fmicb.2021.666039 Text en Copyright © 2021 Lindenberg, Lützhøft, Krych, Fielden, Kot, Frøkiær, van Galen, Nielsen and Hansen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lindenberg, Frederikke Christine
Lützhøft, Ditte Olsen
Krych, Lukasz
Fielden, James
Kot, Witold
Frøkiær, Hanne
van Galen, Gaby
Nielsen, Dennis Sandris
Hansen, Axel Kornerup
An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title_full An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title_fullStr An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title_full_unstemmed An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title_short An Oligosaccharide Rich Diet Increases Akkermansia spp. Bacteria in the Equine Microbiota
title_sort oligosaccharide rich diet increases akkermansia spp. bacteria in the equine microbiota
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176217/
https://www.ncbi.nlm.nih.gov/pubmed/34093482
http://dx.doi.org/10.3389/fmicb.2021.666039
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