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MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2

MicroRNAs (miRNAs) are small, noncoding RNAs which can bind to target mRNAs and regulate gene expression. Increasing evidences suggest that miRNAs play an important role in driving hepatocellular carcinoma (HCC) progression by regulating tumor cell proliferation, apoptosis, invasion, and migration....

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Autores principales: Yang, XueMei, Sun, JiaLing, Sun, HaiTao, Wen, Bin, Zhang, MingJia, An, HaiYan, Chen, WeiCong, Zhao, WenTing, Zhong, XiaoDan, He, ChunYu, Pang, Jie, He, SongQi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176251/
https://www.ncbi.nlm.nih.gov/pubmed/34093801
http://dx.doi.org/10.7150/jca.52298
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author Yang, XueMei
Sun, JiaLing
Sun, HaiTao
Wen, Bin
Zhang, MingJia
An, HaiYan
Chen, WeiCong
Zhao, WenTing
Zhong, XiaoDan
He, ChunYu
Pang, Jie
He, SongQi
author_facet Yang, XueMei
Sun, JiaLing
Sun, HaiTao
Wen, Bin
Zhang, MingJia
An, HaiYan
Chen, WeiCong
Zhao, WenTing
Zhong, XiaoDan
He, ChunYu
Pang, Jie
He, SongQi
author_sort Yang, XueMei
collection PubMed
description MicroRNAs (miRNAs) are small, noncoding RNAs which can bind to target mRNAs and regulate gene expression. Increasing evidences suggest that miRNAs play an important role in driving hepatocellular carcinoma (HCC) progression by regulating tumor cell proliferation, apoptosis, invasion, and migration. In this study, we demonstrated that the expression of microRNA-30a-3p (miR-30a-3p) was reduced in HCC cell lines in comparison to immortalized liver cell line, LO2. Augmented miR-30a-3p level markedly inhibited MHCC-97H cell growth, migration and invasion in vitro. MiR-30a-3p was also found to inhibit tumor growth in vivo using tumor-bearing mice. Mechanismly, COX-2 was discovered to be a direct and functional target of miR-30a-3p in MHCC-97H cells. Raised miR-30a-3p expression reduced the transcriptional level of COX-2 in MHCC-97H cells, while genetically upregulated COX-2 expression was able to reverse the function of miR-30a-3p-mediated suppression of MHCC-97H cells growth, migration and invasion. In addition, we found that using a COX-2 inhibitor, celecoxib, could enhance the anti-metastatic role of miR-30a-3p in MHCC-97H cells. Lastly, we found that decreased COX-2 protein level affected PGE2 production, leading to lower Bcl-2, Caspase-3, MMP2 and MMP9 expression but higher Bax and E-cadherin expression, which in turn culminated in higher rates of cell death and lower rates of cell migration. Taken together, our findings demonstrate that miR-30a-3p could be a target for the treatment of hepatocellular carcinoma cells progression.
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spelling pubmed-81762512021-06-04 MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2 Yang, XueMei Sun, JiaLing Sun, HaiTao Wen, Bin Zhang, MingJia An, HaiYan Chen, WeiCong Zhao, WenTing Zhong, XiaoDan He, ChunYu Pang, Jie He, SongQi J Cancer Research Paper MicroRNAs (miRNAs) are small, noncoding RNAs which can bind to target mRNAs and regulate gene expression. Increasing evidences suggest that miRNAs play an important role in driving hepatocellular carcinoma (HCC) progression by regulating tumor cell proliferation, apoptosis, invasion, and migration. In this study, we demonstrated that the expression of microRNA-30a-3p (miR-30a-3p) was reduced in HCC cell lines in comparison to immortalized liver cell line, LO2. Augmented miR-30a-3p level markedly inhibited MHCC-97H cell growth, migration and invasion in vitro. MiR-30a-3p was also found to inhibit tumor growth in vivo using tumor-bearing mice. Mechanismly, COX-2 was discovered to be a direct and functional target of miR-30a-3p in MHCC-97H cells. Raised miR-30a-3p expression reduced the transcriptional level of COX-2 in MHCC-97H cells, while genetically upregulated COX-2 expression was able to reverse the function of miR-30a-3p-mediated suppression of MHCC-97H cells growth, migration and invasion. In addition, we found that using a COX-2 inhibitor, celecoxib, could enhance the anti-metastatic role of miR-30a-3p in MHCC-97H cells. Lastly, we found that decreased COX-2 protein level affected PGE2 production, leading to lower Bcl-2, Caspase-3, MMP2 and MMP9 expression but higher Bax and E-cadherin expression, which in turn culminated in higher rates of cell death and lower rates of cell migration. Taken together, our findings demonstrate that miR-30a-3p could be a target for the treatment of hepatocellular carcinoma cells progression. Ivyspring International Publisher 2021-05-10 /pmc/articles/PMC8176251/ /pubmed/34093801 http://dx.doi.org/10.7150/jca.52298 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Yang, XueMei
Sun, JiaLing
Sun, HaiTao
Wen, Bin
Zhang, MingJia
An, HaiYan
Chen, WeiCong
Zhao, WenTing
Zhong, XiaoDan
He, ChunYu
Pang, Jie
He, SongQi
MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title_full MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title_fullStr MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title_full_unstemmed MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title_short MicroRNA-30a-3p acts as a tumor suppressor in MHCC-97H hepatocellular carcinoma cells by targeting COX-2
title_sort microrna-30a-3p acts as a tumor suppressor in mhcc-97h hepatocellular carcinoma cells by targeting cox-2
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176251/
https://www.ncbi.nlm.nih.gov/pubmed/34093801
http://dx.doi.org/10.7150/jca.52298
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