Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing

Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for ti...

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Detalles Bibliográficos
Autores principales: Lee, Seongsin, Kim, Yumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176364/
https://www.ncbi.nlm.nih.gov/pubmed/34136832
http://dx.doi.org/10.1016/j.xpro.2021.100564
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author Lee, Seongsin
Kim, Yumi
author_facet Lee, Seongsin
Kim, Yumi
author_sort Lee, Seongsin
collection PubMed
description Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing. For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021).
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spelling pubmed-81763642021-06-15 Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing Lee, Seongsin Kim, Yumi STAR Protoc Protocol Distribution of cells and proteins in whole organs, such as the brain, can provide another level of insight into molecular and cellular functions. Here, we describe a whole-brain immunostaining method using the turquoise killifish, an emerging model for aging research. We optimized a protocol for tissue clearing and whole-brain immunostaining to the turquoise killifish brain. This protocol provides a comprehensive procedure from brain dissection to whole-brain imaging and image processing. For complete details on the use and execution of this protocol, please refer to Eunjeong Do (2020) and Lee et al. (2021). Elsevier 2021-05-26 /pmc/articles/PMC8176364/ /pubmed/34136832 http://dx.doi.org/10.1016/j.xpro.2021.100564 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Lee, Seongsin
Kim, Yumi
Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title_full Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title_fullStr Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title_full_unstemmed Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title_short Protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
title_sort protocol for whole-brain immunostaining of the turquoise killifish after tissue clearing
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176364/
https://www.ncbi.nlm.nih.gov/pubmed/34136832
http://dx.doi.org/10.1016/j.xpro.2021.100564
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