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Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts

Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol...

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Detalles Bibliográficos
Autores principales: Yasuda, Tadahito, Koiwa, Mayu, Yonemura, Atsuko, Akiyama, Takahiko, Baba, Hideo, Ishimoto, Takatsugu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176369/
https://www.ncbi.nlm.nih.gov/pubmed/34136831
http://dx.doi.org/10.1016/j.xpro.2021.100553