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Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2
To increase the repertoire of PCR based laboratory developed tests (LDTs) for the detection of SARS-CoV-2, we describe a new multiplex assay (SORP), targeting the SARS-CoV-2's, Spike and ORF8 genes. The widely used human RNaseP internal control was modified to specifically co-amplify the RNaseP...
Autores principales: | Gadkar, Vijay J., Goldfarb, David M., Young, Virginia, Watson, Nicole, Al-Rawahi, Ghada N., Srigley, Jocelyn A., Tilley, Peter |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8176879/ https://www.ncbi.nlm.nih.gov/pubmed/34089805 http://dx.doi.org/10.1016/j.mcp.2021.101744 |
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