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Comparison of simple and rapid extracting methods of free-tags Mycobacterium tuberculosis protein 64 Recombinant Protein from polyacrylamide gel: Electroelution and the optimized passive elution

In this study, the Mycobacterium tuberculosis protein 64 (MPT64) protein was constructed without any tags to facilitate the purification using column affinity chromatography, but the MPT64 must be obtained as a pure protein. This study was purpose to ensure the efficient extracting method to purify...

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Detalles Bibliográficos
Autores principales: Kusuma, Sri Agung Fitri, Parwati, Ida, Subroto, Toto, Rukayadi, Yaya, Fadhlillah, Muhammad, Rizaludin, Asep
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177148/
https://www.ncbi.nlm.nih.gov/pubmed/34159151
http://dx.doi.org/10.4103/japtr.JAPTR_318_20