Cargando…

Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw

The egg white was used to induce rat paw inflammation, with inadequate references to explain its mechanism. It's contained protein was identified as an allergen was suspected to trigger an inflammatory reaction. This research was aimed to evaluate the use of egg white as an inflammatory inducto...

Descripción completa

Detalles Bibliográficos
Autores principales: Barung, Elisabeth Natalia, Dumanauw, Jovie Mien, Duri, Meilany Feronika, Kalonio, Donald Emilio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177150/
https://www.ncbi.nlm.nih.gov/pubmed/34159139
http://dx.doi.org/10.4103/japtr.JAPTR_262_20
_version_ 1783703355890073600
author Barung, Elisabeth Natalia
Dumanauw, Jovie Mien
Duri, Meilany Feronika
Kalonio, Donald Emilio
author_facet Barung, Elisabeth Natalia
Dumanauw, Jovie Mien
Duri, Meilany Feronika
Kalonio, Donald Emilio
author_sort Barung, Elisabeth Natalia
collection PubMed
description The egg white was used to induce rat paw inflammation, with inadequate references to explain its mechanism. It's contained protein was identified as an allergen was suspected to trigger an inflammatory reaction. This research was aimed to evaluate the use of egg white as an inflammatory inductor in inflammation animal models through edema profile and histological change. Male Wistar rats were divided into three groups, which were given λ-carrageenan, fresh takes of the hen's egg white, and sterile saline solution. Edema was induced by subcutaneous injection of 0.1 ml of λ-carrageenan (1%), egg white, and sterile saline solution as the control in the hind paw of rats. Paw volume was measured before and then at 1, 2, 3, 4, 5, 6, and 24 h after the inductor injection. Paw tissue was taken for evaluation of rats’ paw histological change. The data were analyzed by one-way ANOVA followed by LSD test. The results of the study showed that the egg white could induce rat paw inflammation. Edema formation began in the 1(st) h and reached the peaks in the 2(nd) h after the subcutaneous injection of egg white. A number of leukocyte cells were also found in the inflamed paw tissues. Egg white was potential as an edema inductor for animal models of inflammation for the evaluation of new drugs or natural product with anti-inflammation activity.
format Online
Article
Text
id pubmed-8177150
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Wolters Kluwer - Medknow
record_format MEDLINE/PubMed
spelling pubmed-81771502021-06-21 Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw Barung, Elisabeth Natalia Dumanauw, Jovie Mien Duri, Meilany Feronika Kalonio, Donald Emilio J Adv Pharm Technol Res Original Article The egg white was used to induce rat paw inflammation, with inadequate references to explain its mechanism. It's contained protein was identified as an allergen was suspected to trigger an inflammatory reaction. This research was aimed to evaluate the use of egg white as an inflammatory inductor in inflammation animal models through edema profile and histological change. Male Wistar rats were divided into three groups, which were given λ-carrageenan, fresh takes of the hen's egg white, and sterile saline solution. Edema was induced by subcutaneous injection of 0.1 ml of λ-carrageenan (1%), egg white, and sterile saline solution as the control in the hind paw of rats. Paw volume was measured before and then at 1, 2, 3, 4, 5, 6, and 24 h after the inductor injection. Paw tissue was taken for evaluation of rats’ paw histological change. The data were analyzed by one-way ANOVA followed by LSD test. The results of the study showed that the egg white could induce rat paw inflammation. Edema formation began in the 1(st) h and reached the peaks in the 2(nd) h after the subcutaneous injection of egg white. A number of leukocyte cells were also found in the inflamed paw tissues. Egg white was potential as an edema inductor for animal models of inflammation for the evaluation of new drugs or natural product with anti-inflammation activity. Wolters Kluwer - Medknow 2021 2021-04-27 /pmc/articles/PMC8177150/ /pubmed/34159139 http://dx.doi.org/10.4103/japtr.JAPTR_262_20 Text en Copyright: © 2021 Journal of Advanced Pharmaceutical Technology & Research https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Barung, Elisabeth Natalia
Dumanauw, Jovie Mien
Duri, Meilany Feronika
Kalonio, Donald Emilio
Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title_full Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title_fullStr Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title_full_unstemmed Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title_short Egg white-induced inflammation models: A study of edema profile and histological change of rat's paw
title_sort egg white-induced inflammation models: a study of edema profile and histological change of rat's paw
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177150/
https://www.ncbi.nlm.nih.gov/pubmed/34159139
http://dx.doi.org/10.4103/japtr.JAPTR_262_20
work_keys_str_mv AT barungelisabethnatalia eggwhiteinducedinflammationmodelsastudyofedemaprofileandhistologicalchangeofratspaw
AT dumanauwjoviemien eggwhiteinducedinflammationmodelsastudyofedemaprofileandhistologicalchangeofratspaw
AT durimeilanyferonika eggwhiteinducedinflammationmodelsastudyofedemaprofileandhistologicalchangeofratspaw
AT kaloniodonaldemilio eggwhiteinducedinflammationmodelsastudyofedemaprofileandhistologicalchangeofratspaw