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Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer

Peripheral regulatory T cells (pTregs) are a highly immunosuppressive fraction of CD4+ T cells. We aimed to evaluate the clinical significance of pTregs in patients with gastric cancer and to determine the correlation between pTregs and immune cell infiltration in tumor microenvironment. pTregs stat...

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Autores principales: Suh, Koung Jin, Kim, Jin Won, Kim, Ji Eun, Sung, Ji Hea, Koh, Jiwon, Kim, Kui-Jin, Kim, Ji-Won, Ahn, Sang-Hoon, Park, Do Joong, Kim, Hyung-Ho, Lee, Hye Seung, Lee, Keun-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177409/
https://www.ncbi.nlm.nih.gov/pubmed/34086741
http://dx.doi.org/10.1371/journal.pone.0252480
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author Suh, Koung Jin
Kim, Jin Won
Kim, Ji Eun
Sung, Ji Hea
Koh, Jiwon
Kim, Kui-Jin
Kim, Ji-Won
Ahn, Sang-Hoon
Park, Do Joong
Kim, Hyung-Ho
Lee, Hye Seung
Lee, Keun-Wook
author_facet Suh, Koung Jin
Kim, Jin Won
Kim, Ji Eun
Sung, Ji Hea
Koh, Jiwon
Kim, Kui-Jin
Kim, Ji-Won
Ahn, Sang-Hoon
Park, Do Joong
Kim, Hyung-Ho
Lee, Hye Seung
Lee, Keun-Wook
author_sort Suh, Koung Jin
collection PubMed
description Peripheral regulatory T cells (pTregs) are a highly immunosuppressive fraction of CD4+ T cells. We aimed to evaluate the clinical significance of pTregs in patients with gastric cancer and to determine the correlation between pTregs and immune cell infiltration in tumor microenvironment. pTregs status was determined by assessing the pTreg/total T-cell ratio (ratio of Foxp3 Treg-specific demethylated region (TSDR) to CD3G/CD3D demethylation, so-called Cellular Ratio of Immune Tolerance “ImmunoCRIT”) using methylation analyses in 433 patients with gastric cancer who received curative surgery. Among 422 evaluable patients, 230 (54.5%) had high ImmunoCRIT (> 21.0). Patients with high ImmunoCRIT had significantly shorter disease-free survival (DFS) and overall survival (OS) than those with high ImmunoCRIT (p = 0.030, p = 0.008, respectively). In multivariate analysis, high ImmunoCRIT kept a prognostic role for shorter OS (hazard ratio [HR] 1.9, 95% confidence interval [CI] 1.4–2.9; p = 0.005). CD3+ cell density and CD4+ cell density was significantly higher within the tumor in high ImmunoCRIT group than those in low ImmunoCRIT group (CD3+ cell, 202.12/mm(2) vs. 172.2/mm(2), p = 0.029; CD4+ cell, 56.5/mm(2) vs. 43.5/mm(2), p = 0.007). In conclusion, the peripheral ImmunoCRIT determined by epigenetic methylation analysis provides prognostic information in resected gastric tumors.
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spelling pubmed-81774092021-06-07 Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer Suh, Koung Jin Kim, Jin Won Kim, Ji Eun Sung, Ji Hea Koh, Jiwon Kim, Kui-Jin Kim, Ji-Won Ahn, Sang-Hoon Park, Do Joong Kim, Hyung-Ho Lee, Hye Seung Lee, Keun-Wook PLoS One Research Article Peripheral regulatory T cells (pTregs) are a highly immunosuppressive fraction of CD4+ T cells. We aimed to evaluate the clinical significance of pTregs in patients with gastric cancer and to determine the correlation between pTregs and immune cell infiltration in tumor microenvironment. pTregs status was determined by assessing the pTreg/total T-cell ratio (ratio of Foxp3 Treg-specific demethylated region (TSDR) to CD3G/CD3D demethylation, so-called Cellular Ratio of Immune Tolerance “ImmunoCRIT”) using methylation analyses in 433 patients with gastric cancer who received curative surgery. Among 422 evaluable patients, 230 (54.5%) had high ImmunoCRIT (> 21.0). Patients with high ImmunoCRIT had significantly shorter disease-free survival (DFS) and overall survival (OS) than those with high ImmunoCRIT (p = 0.030, p = 0.008, respectively). In multivariate analysis, high ImmunoCRIT kept a prognostic role for shorter OS (hazard ratio [HR] 1.9, 95% confidence interval [CI] 1.4–2.9; p = 0.005). CD3+ cell density and CD4+ cell density was significantly higher within the tumor in high ImmunoCRIT group than those in low ImmunoCRIT group (CD3+ cell, 202.12/mm(2) vs. 172.2/mm(2), p = 0.029; CD4+ cell, 56.5/mm(2) vs. 43.5/mm(2), p = 0.007). In conclusion, the peripheral ImmunoCRIT determined by epigenetic methylation analysis provides prognostic information in resected gastric tumors. Public Library of Science 2021-06-04 /pmc/articles/PMC8177409/ /pubmed/34086741 http://dx.doi.org/10.1371/journal.pone.0252480 Text en © 2021 Suh et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Suh, Koung Jin
Kim, Jin Won
Kim, Ji Eun
Sung, Ji Hea
Koh, Jiwon
Kim, Kui-Jin
Kim, Ji-Won
Ahn, Sang-Hoon
Park, Do Joong
Kim, Hyung-Ho
Lee, Hye Seung
Lee, Keun-Wook
Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title_full Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title_fullStr Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title_full_unstemmed Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title_short Correlation between tumor infiltrating immune cells and peripheral regulatory T cell determined using methylation analyses and its prognostic significance in resected gastric cancer
title_sort correlation between tumor infiltrating immune cells and peripheral regulatory t cell determined using methylation analyses and its prognostic significance in resected gastric cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177409/
https://www.ncbi.nlm.nih.gov/pubmed/34086741
http://dx.doi.org/10.1371/journal.pone.0252480
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