Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire

Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proli...

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Autores principales: Dill, Veronika, Pfaff, Florian, Zimmer, Aline, Beer, Martin, Eschbaumer, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177424/
https://www.ncbi.nlm.nih.gov/pubmed/34086711
http://dx.doi.org/10.1371/journal.pone.0246610
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author Dill, Veronika
Pfaff, Florian
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
author_facet Dill, Veronika
Pfaff, Florian
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
author_sort Dill, Veronika
collection PubMed
description Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proliferate in suspension to high cell numbers. The molecular mechanisms that facilitate the adaptation and that take place inside the cell are largely unknown. Especially for cell lines that are used for virus antigen production such as baby hamster kidney (BHK) cells, the restriction of virus growth through the evolution of undesired cell characteristics is highly unwanted. The comparison between adherently growing BHK cells and suspension cells with different susceptibility to foot-and-mouth disease virus revealed differences in the expression of cellular receptors such as integrins and heparan sulfates, and in the organization of the actin cytoskeleton. Transcriptome analyses and growth kinetics demonstrated the diversity of BHK cell lines and confirmed the importance of well-characterized parental cell clones and mindful screening to make sure that essential cellular features do not get lost during adaptation.
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spelling pubmed-81774242021-06-07 Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire Dill, Veronika Pfaff, Florian Zimmer, Aline Beer, Martin Eschbaumer, Michael PLoS One Research Article Animal cell culture, with single cells growing in suspension, ideally in a chemically defined environment, is a mainstay of biopharmaceutical production. The synthetic environment lacks exogenous growth factors and usually requires a time-consuming adaptation process to select cell clones that proliferate in suspension to high cell numbers. The molecular mechanisms that facilitate the adaptation and that take place inside the cell are largely unknown. Especially for cell lines that are used for virus antigen production such as baby hamster kidney (BHK) cells, the restriction of virus growth through the evolution of undesired cell characteristics is highly unwanted. The comparison between adherently growing BHK cells and suspension cells with different susceptibility to foot-and-mouth disease virus revealed differences in the expression of cellular receptors such as integrins and heparan sulfates, and in the organization of the actin cytoskeleton. Transcriptome analyses and growth kinetics demonstrated the diversity of BHK cell lines and confirmed the importance of well-characterized parental cell clones and mindful screening to make sure that essential cellular features do not get lost during adaptation. Public Library of Science 2021-06-04 /pmc/articles/PMC8177424/ /pubmed/34086711 http://dx.doi.org/10.1371/journal.pone.0246610 Text en © 2021 Dill et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dill, Veronika
Pfaff, Florian
Zimmer, Aline
Beer, Martin
Eschbaumer, Michael
Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title_full Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title_fullStr Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title_full_unstemmed Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title_short Adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
title_sort adherent and suspension baby hamster kidney cells have a different cytoskeleton and surface receptor repertoire
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177424/
https://www.ncbi.nlm.nih.gov/pubmed/34086711
http://dx.doi.org/10.1371/journal.pone.0246610
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