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A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells

BACKGROUND: Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many types of cancer, inc...

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Autores principales: Zhu, Lili, Cui, Kang, Weng, Lanling, Yu, Pu, Du, Yabing, Zhang, Tengfei, Liu, Hong, Li, Bingjie, Ma, Wang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177463/
https://www.ncbi.nlm.nih.gov/pubmed/34086679
http://dx.doi.org/10.1371/journal.pone.0249174
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author Zhu, Lili
Cui, Kang
Weng, Lanling
Yu, Pu
Du, Yabing
Zhang, Tengfei
Liu, Hong
Li, Bingjie
Ma, Wang
author_facet Zhu, Lili
Cui, Kang
Weng, Lanling
Yu, Pu
Du, Yabing
Zhang, Tengfei
Liu, Hong
Li, Bingjie
Ma, Wang
author_sort Zhu, Lili
collection PubMed
description BACKGROUND: Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many types of cancer, including pancreatic adenocarcinoma, gastric cancer, liver cancer, and lung cancer. Previous studies have shown that many lncRNAs are associated with the occurrence and development of BCa. However, few studies have combined multiple lncRNAs to predict the prognosis of early-stage BCa patients. METHODS: Systematic and comprehensive analysis of data from The Cancer Genome Atlas (TCGA) was conducted to identify lncRNA signatures with prognostic value in BCa. Additionally, the relative expression levels of the 8 lncRNA of several BCa cell lines were detected by quantitative real-time PCR (qPCR) and the results were substituted into a risk score formula. Finally, migration assays were used to verify the result from prognostic analysis according to the risk scores among cell lines with different risk scores. RESULTS: Our study included 808 BCa patients with complete clinical data. A panel of 8 lncRNAs was identified using Wilcox tests as different between normal and tumor tissue of the BCa patients. This panel was used to analyze the survival of BCa patients. Patients with low risk scores had greater overall survival (OS) than those with high risk scores. Multivariate Cox regression analyses demonstrated that the lncRNA signature was an independent prognostic factor. Gene Set Enrichment Analysis (GSEA) suggested that the lncRNAs might be involved in several molecular signaling pathways implicated in BCa such as the DNA replication pathway, the cell cycle pathway, and the pentose phosphate pathway. Validation experiments in breast cancer cells to test cell migration by using wound-healing assays supported the results of the model. CONCLUSION: Our study demonstrated that a panel of 8 lncRNAs has the potential to be used as an independent prognostic biomarker of BCa.
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spelling pubmed-81774632021-06-07 A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells Zhu, Lili Cui, Kang Weng, Lanling Yu, Pu Du, Yabing Zhang, Tengfei Liu, Hong Li, Bingjie Ma, Wang PLoS One Research Article BACKGROUND: Breast cancer (BCa) is the most commonly diagnosed cancer and the leading cause of cancer death among females around the world. Recent studies have indicated that long non-coding RNAs (lncRNAs) can serve as an independent biomarker for diagnosis and prognosis in many types of cancer, including pancreatic adenocarcinoma, gastric cancer, liver cancer, and lung cancer. Previous studies have shown that many lncRNAs are associated with the occurrence and development of BCa. However, few studies have combined multiple lncRNAs to predict the prognosis of early-stage BCa patients. METHODS: Systematic and comprehensive analysis of data from The Cancer Genome Atlas (TCGA) was conducted to identify lncRNA signatures with prognostic value in BCa. Additionally, the relative expression levels of the 8 lncRNA of several BCa cell lines were detected by quantitative real-time PCR (qPCR) and the results were substituted into a risk score formula. Finally, migration assays were used to verify the result from prognostic analysis according to the risk scores among cell lines with different risk scores. RESULTS: Our study included 808 BCa patients with complete clinical data. A panel of 8 lncRNAs was identified using Wilcox tests as different between normal and tumor tissue of the BCa patients. This panel was used to analyze the survival of BCa patients. Patients with low risk scores had greater overall survival (OS) than those with high risk scores. Multivariate Cox regression analyses demonstrated that the lncRNA signature was an independent prognostic factor. Gene Set Enrichment Analysis (GSEA) suggested that the lncRNAs might be involved in several molecular signaling pathways implicated in BCa such as the DNA replication pathway, the cell cycle pathway, and the pentose phosphate pathway. Validation experiments in breast cancer cells to test cell migration by using wound-healing assays supported the results of the model. CONCLUSION: Our study demonstrated that a panel of 8 lncRNAs has the potential to be used as an independent prognostic biomarker of BCa. Public Library of Science 2021-06-04 /pmc/articles/PMC8177463/ /pubmed/34086679 http://dx.doi.org/10.1371/journal.pone.0249174 Text en © 2021 Zhu et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhu, Lili
Cui, Kang
Weng, Lanling
Yu, Pu
Du, Yabing
Zhang, Tengfei
Liu, Hong
Li, Bingjie
Ma, Wang
A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title_full A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title_fullStr A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title_full_unstemmed A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title_short A panel of 8-lncRNA predicts prognosis of breast cancer patients and migration of breast cancer cells
title_sort panel of 8-lncrna predicts prognosis of breast cancer patients and migration of breast cancer cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177463/
https://www.ncbi.nlm.nih.gov/pubmed/34086679
http://dx.doi.org/10.1371/journal.pone.0249174
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