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Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step

The use of personal protective equipment (PPE) has been considered the most effective way to avoid the contamination of healthcare workers by different microorganisms, including SARS-CoV-2. A spray disinfection technology (chamber) was developed, and its efficacy in instant decontamination of previo...

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Autores principales: Brêda Mascarenhas, Luís Alberto, Machado, Bruna Aparecida Souza, Rodrigues, Leticia de Alencar Pereira, Saraiva Hodel, Katharine Valéria, Bandeira Santos, Alex Álisson, Freitas Neves, Paulo Roberto, da Silva Andrade, Leone Peter Correia, Soares, Milena Botelho, de Andrade, Jailson Bittencourt, Badaró, Roberto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177472/
https://www.ncbi.nlm.nih.gov/pubmed/34086691
http://dx.doi.org/10.1371/journal.pone.0250854
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author Brêda Mascarenhas, Luís Alberto
Machado, Bruna Aparecida Souza
Rodrigues, Leticia de Alencar Pereira
Saraiva Hodel, Katharine Valéria
Bandeira Santos, Alex Álisson
Freitas Neves, Paulo Roberto
da Silva Andrade, Leone Peter Correia
Soares, Milena Botelho
de Andrade, Jailson Bittencourt
Badaró, Roberto
author_facet Brêda Mascarenhas, Luís Alberto
Machado, Bruna Aparecida Souza
Rodrigues, Leticia de Alencar Pereira
Saraiva Hodel, Katharine Valéria
Bandeira Santos, Alex Álisson
Freitas Neves, Paulo Roberto
da Silva Andrade, Leone Peter Correia
Soares, Milena Botelho
de Andrade, Jailson Bittencourt
Badaró, Roberto
author_sort Brêda Mascarenhas, Luís Alberto
collection PubMed
description The use of personal protective equipment (PPE) has been considered the most effective way to avoid the contamination of healthcare workers by different microorganisms, including SARS-CoV-2. A spray disinfection technology (chamber) was developed, and its efficacy in instant decontamination of previously contaminated surfaces was evaluated in two exposure times. Seven test microorganisms were prepared and inoculated on the surface of seven types of PPE (respirator mask, face shield, shoe, glove, cap, safety glasses and lab coat). The tests were performed on previously contaminated PPE using a manikin with a motion device for exposure to the chamber with biocidal agent (sodium hypochlorite) for 10 and 30s. In 96.93% of the experimental conditions analyzed, the percentage reduction was >99% (the number of viable cells found on the surface ranged from 4.3x10(6) to <10 CFU/mL). The samples of E. faecalis collected from the glove showed the lowest percentages reduction, with 86.000 and 86.500% for exposure times of 10 and 30 s, respectively. The log(10) reduction values varied between 0.85 log(10) (E. faecalis at 30 s in glove surface) and 9.69 log(10) (E. coli at 10 and 30 s in lab coat surface). In general, E. coli, S. aureus, C. freundii, P. mirabilis, C. albicans and C. parapsilosis showed susceptibility to the biocidal agent under the tested conditions, with >99% reduction after 10 and 30s, while E. faecalis and P. aeruginosa showed a lower susceptibility. The 30s exposure time was more effective for the inactivation of the tested microorganisms. The results show that the spray disinfection technology has the potential for instant decontamination of PPE, which can contribute to an additional barrier for infection control of healthcare workers in the hospital environment.
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spelling pubmed-81774722021-06-07 Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step Brêda Mascarenhas, Luís Alberto Machado, Bruna Aparecida Souza Rodrigues, Leticia de Alencar Pereira Saraiva Hodel, Katharine Valéria Bandeira Santos, Alex Álisson Freitas Neves, Paulo Roberto da Silva Andrade, Leone Peter Correia Soares, Milena Botelho de Andrade, Jailson Bittencourt Badaró, Roberto PLoS One Research Article The use of personal protective equipment (PPE) has been considered the most effective way to avoid the contamination of healthcare workers by different microorganisms, including SARS-CoV-2. A spray disinfection technology (chamber) was developed, and its efficacy in instant decontamination of previously contaminated surfaces was evaluated in two exposure times. Seven test microorganisms were prepared and inoculated on the surface of seven types of PPE (respirator mask, face shield, shoe, glove, cap, safety glasses and lab coat). The tests were performed on previously contaminated PPE using a manikin with a motion device for exposure to the chamber with biocidal agent (sodium hypochlorite) for 10 and 30s. In 96.93% of the experimental conditions analyzed, the percentage reduction was >99% (the number of viable cells found on the surface ranged from 4.3x10(6) to <10 CFU/mL). The samples of E. faecalis collected from the glove showed the lowest percentages reduction, with 86.000 and 86.500% for exposure times of 10 and 30 s, respectively. The log(10) reduction values varied between 0.85 log(10) (E. faecalis at 30 s in glove surface) and 9.69 log(10) (E. coli at 10 and 30 s in lab coat surface). In general, E. coli, S. aureus, C. freundii, P. mirabilis, C. albicans and C. parapsilosis showed susceptibility to the biocidal agent under the tested conditions, with >99% reduction after 10 and 30s, while E. faecalis and P. aeruginosa showed a lower susceptibility. The 30s exposure time was more effective for the inactivation of the tested microorganisms. The results show that the spray disinfection technology has the potential for instant decontamination of PPE, which can contribute to an additional barrier for infection control of healthcare workers in the hospital environment. Public Library of Science 2021-06-04 /pmc/articles/PMC8177472/ /pubmed/34086691 http://dx.doi.org/10.1371/journal.pone.0250854 Text en © 2021 Brêda Mascarenhas et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Brêda Mascarenhas, Luís Alberto
Machado, Bruna Aparecida Souza
Rodrigues, Leticia de Alencar Pereira
Saraiva Hodel, Katharine Valéria
Bandeira Santos, Alex Álisson
Freitas Neves, Paulo Roberto
da Silva Andrade, Leone Peter Correia
Soares, Milena Botelho
de Andrade, Jailson Bittencourt
Badaró, Roberto
Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title_full Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title_fullStr Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title_full_unstemmed Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title_short Potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
title_sort potential application of novel technology developed for instant decontamination of personal protective equipment before the doffing step
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177472/
https://www.ncbi.nlm.nih.gov/pubmed/34086691
http://dx.doi.org/10.1371/journal.pone.0250854
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