Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection

The general methods to detect the RNA of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) in clinical diagnostic testing involve reverse transcriptases and thermostable DNA polymerases. In this study, we compared the detection of SARS-CoV-2 by a one-step real-time RT-PCR method using a h...

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Detalles Bibliográficos
Autores principales: Nakura, Yukiko, Wu, Heng Ning, Okamoto, Yuya, Takeuchi, Muneyuki, Suzuki, Koichiro, Tamura, Yoshitaka, Oba, Yuichiro, Nishiumi, Fumiko, Hatori, Nobuaki, Fujiwara, Shinsuke, Yasukawa, Kiyoshi, Ida, Shinobu, Yanagihara, Itaru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177496/
https://www.ncbi.nlm.nih.gov/pubmed/34086827
http://dx.doi.org/10.1371/journal.pone.0252789

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