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Inferring spikes from calcium imaging in dopamine neurons

Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and e...

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Detalles Bibliográficos
Autores principales: Fleming, Weston, Jewell, Sean, Engelhard, Ben, Witten, Daniela M., Witten, Ilana B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177503/
https://www.ncbi.nlm.nih.gov/pubmed/34086726
http://dx.doi.org/10.1371/journal.pone.0252345
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author Fleming, Weston
Jewell, Sean
Engelhard, Ben
Witten, Daniela M.
Witten, Ilana B.
author_facet Fleming, Weston
Jewell, Sean
Engelhard, Ben
Witten, Daniela M.
Witten, Ilana B.
author_sort Fleming, Weston
collection PubMed
description Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and electrophysiology in DA neurons in brain slices and applied a recently developed spike inference algorithm to the GCaMP fluorescence. This revealed that individual spikes can be inferred accurately in this population. Next, we inferred spikes in vivo from calcium imaging from these neurons during Pavlovian conditioning, as well as during navigation in virtual reality. In both cases, we quantitatively recapitulated previous in vivo electrophysiological observations. Our work provides a validated approach to infer spikes from calcium imaging in DA neurons and implies that aspects of both tonic and phasic spike patterns can be recovered.
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spelling pubmed-81775032021-06-07 Inferring spikes from calcium imaging in dopamine neurons Fleming, Weston Jewell, Sean Engelhard, Ben Witten, Daniela M. Witten, Ilana B. PLoS One Research Article Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and electrophysiology in DA neurons in brain slices and applied a recently developed spike inference algorithm to the GCaMP fluorescence. This revealed that individual spikes can be inferred accurately in this population. Next, we inferred spikes in vivo from calcium imaging from these neurons during Pavlovian conditioning, as well as during navigation in virtual reality. In both cases, we quantitatively recapitulated previous in vivo electrophysiological observations. Our work provides a validated approach to infer spikes from calcium imaging in DA neurons and implies that aspects of both tonic and phasic spike patterns can be recovered. Public Library of Science 2021-06-04 /pmc/articles/PMC8177503/ /pubmed/34086726 http://dx.doi.org/10.1371/journal.pone.0252345 Text en © 2021 Fleming et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Fleming, Weston
Jewell, Sean
Engelhard, Ben
Witten, Daniela M.
Witten, Ilana B.
Inferring spikes from calcium imaging in dopamine neurons
title Inferring spikes from calcium imaging in dopamine neurons
title_full Inferring spikes from calcium imaging in dopamine neurons
title_fullStr Inferring spikes from calcium imaging in dopamine neurons
title_full_unstemmed Inferring spikes from calcium imaging in dopamine neurons
title_short Inferring spikes from calcium imaging in dopamine neurons
title_sort inferring spikes from calcium imaging in dopamine neurons
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177503/
https://www.ncbi.nlm.nih.gov/pubmed/34086726
http://dx.doi.org/10.1371/journal.pone.0252345
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