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Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress

BACKGROUND: The seizures, triggered by a loud sound in laboratory animals, are associated with the imbalance of the brain monoamines. Serotonin (5-HT) is regarded as one of the principal neurotransmitters to be involved in regulation of wide variety of physiological and psychological processes. Amon...

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Autores principales: Ismayilova, Khadija, Hashimova, Ulduz, Majidi, Mohammad Reza, Rustamov, Farhad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Whioce Publishing Pte. Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177837/
https://www.ncbi.nlm.nih.gov/pubmed/34104832
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author Ismayilova, Khadija
Hashimova, Ulduz
Majidi, Mohammad Reza
Rustamov, Farhad
author_facet Ismayilova, Khadija
Hashimova, Ulduz
Majidi, Mohammad Reza
Rustamov, Farhad
author_sort Ismayilova, Khadija
collection PubMed
description BACKGROUND: The seizures, triggered by a loud sound in laboratory animals, are associated with the imbalance of the brain monoamines. Serotonin (5-HT) is regarded as one of the principal neurotransmitters to be involved in regulation of wide variety of physiological and psychological processes. Among the drugs that affect 5-HT synaptic transmission, the leading role is given to selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine. AIM: The aim of the study is to investigate the effects of fluoxetine on seizures in Wistar rats with high susceptibility to audiogenic stress. METHODS: The study was performed on male Wistar rats. Before the experiments, the animals were tested for susceptibility to audiogenic seizures by exposure to a sound of 90–110 dB for 2 min in the soundproof box. The difference between congenital susceptibility to seizures served as a basis for dividing animals into two groups: Seizure-susceptible (SS) and seizure-tolerant (ST) rats. One hour before the experiment, the experimental animals were orally administered with fluoxetine, while the control rats were treated with distilled water. The effect of fluoxetine on the level of the biogenic amines in different regions of the brain was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: It has been shown that in 100% of the cases, the control SS rats, exposed to audiogenic stimulus, exhibited wild running around in circles and jumping as some of the signs of seizure responses that evolved into tonic–clonic seizures in 70% of the cases. As regards the experimental SS animals: Only in 60% of the cases (P<0.05), they exhibited wild running and flinch, which did not evolve into the tonic–clonic phase. CONCLUSION: Decreased manifestation of seizures in the experimental SS rats under acute administration of fluoxetine may be explained by highly distinctive repertoire and spatial distribution of 5-HT receptors in their brain structures in comparison to the ST rats. RELEVANCE FOR PATIENTS: The study of the monoaminergic mechanisms of seizure generation is of practical importance due to the existence of pharmacological agents, which selectively affect the activity of the monoaminergic systems of the brain, as well as the metabolism of the monoamines synthesized by them.
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spelling pubmed-81778372021-06-07 Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress Ismayilova, Khadija Hashimova, Ulduz Majidi, Mohammad Reza Rustamov, Farhad J Clin Transl Res Original Article BACKGROUND: The seizures, triggered by a loud sound in laboratory animals, are associated with the imbalance of the brain monoamines. Serotonin (5-HT) is regarded as one of the principal neurotransmitters to be involved in regulation of wide variety of physiological and psychological processes. Among the drugs that affect 5-HT synaptic transmission, the leading role is given to selective serotonin reuptake inhibitors (SSRIs), such as fluoxetine. AIM: The aim of the study is to investigate the effects of fluoxetine on seizures in Wistar rats with high susceptibility to audiogenic stress. METHODS: The study was performed on male Wistar rats. Before the experiments, the animals were tested for susceptibility to audiogenic seizures by exposure to a sound of 90–110 dB for 2 min in the soundproof box. The difference between congenital susceptibility to seizures served as a basis for dividing animals into two groups: Seizure-susceptible (SS) and seizure-tolerant (ST) rats. One hour before the experiment, the experimental animals were orally administered with fluoxetine, while the control rats were treated with distilled water. The effect of fluoxetine on the level of the biogenic amines in different regions of the brain was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: It has been shown that in 100% of the cases, the control SS rats, exposed to audiogenic stimulus, exhibited wild running around in circles and jumping as some of the signs of seizure responses that evolved into tonic–clonic seizures in 70% of the cases. As regards the experimental SS animals: Only in 60% of the cases (P<0.05), they exhibited wild running and flinch, which did not evolve into the tonic–clonic phase. CONCLUSION: Decreased manifestation of seizures in the experimental SS rats under acute administration of fluoxetine may be explained by highly distinctive repertoire and spatial distribution of 5-HT receptors in their brain structures in comparison to the ST rats. RELEVANCE FOR PATIENTS: The study of the monoaminergic mechanisms of seizure generation is of practical importance due to the existence of pharmacological agents, which selectively affect the activity of the monoaminergic systems of the brain, as well as the metabolism of the monoamines synthesized by them. Whioce Publishing Pte. Ltd. 2021-04-22 /pmc/articles/PMC8177837/ /pubmed/34104832 Text en Copyright: © Whioce Publishing Pte. Ltd. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC-ND 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Ismayilova, Khadija
Hashimova, Ulduz
Majidi, Mohammad Reza
Rustamov, Farhad
Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title_full Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title_fullStr Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title_full_unstemmed Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title_short Effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
title_sort effect of fluoxetine on seizures in rats with high susceptibility to audiogenic stress
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8177837/
https://www.ncbi.nlm.nih.gov/pubmed/34104832
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