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Protocol for analysis of G2/M DNA synthesis in human cells

G2/M DNA synthesis (G-MiDS) can be observed in one in five G2/M cells in unperturbed conditions by immunofluorescence microscopy. However, little is known of the genomic sites undergoing G-MiDS. Here, we describe a protocol which allows enriching for G2/M cells and investigating the sites of G-MiDS...

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Detalles Bibliográficos
Autores principales: Wang, Jianming, Saponaro, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178111/
https://www.ncbi.nlm.nih.gov/pubmed/34136835
http://dx.doi.org/10.1016/j.xpro.2021.100570

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