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Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel
A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammat...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178319/ https://www.ncbi.nlm.nih.gov/pubmed/34088942 http://dx.doi.org/10.1038/s41598-021-91109-2 |
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author | Farooq, Amina Butt, Nauman Z. Hassan, Umer |
author_facet | Farooq, Amina Butt, Nauman Z. Hassan, Umer |
author_sort | Farooq, Amina |
collection | PubMed |
description | A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top–bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top–bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in a microfluidic channel in the future. |
format | Online Article Text |
id | pubmed-8178319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81783192021-06-07 Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel Farooq, Amina Butt, Nauman Z. Hassan, Umer Sci Rep Article A biosensor capable of differentiating cells or other microparticles based on morphology finds significant biomedical applications. Examples may include morphological determination in the cellular division process, differentiation of bacterial cells, and cellular morphological variation in inflammation and cancer etc. Here, we present a novel integrated multi-planar microelectrodes geometry design that can distinguish a non-spherical individual particle flowing along a microchannel based on its electrical signature. We simulated multi-planar electrodes design in COMSOL Multiphysics and have shown that the changes in electrical field intensity corresponding to multiple particle morphologies can be distinguished. Our initial investigation has shown that top–bottom electrodes configuration produces significantly enhanced signal strength for a spherical particle as compared to co-planar configuration. Next, we integrated the co-planar and top–bottom configurations to develop a multi-planar microelectrode design capable of electrical impedance measurement at different spatial planes inside a microchannel by collecting multiple output signatures. We tested our integrated multi-planar electrode design with particles of different elliptical morphologies by gradually changing spherical particle dimensions to the non-spherical. The computed electrical signal ratio of non-spherical to spherical particle shows a very good correlation to predict the particle morphology. The biochip sensitivity is also found be independent of orientation of the particle flowing in the microchannel. Our integrated design will help develop the technology that will allow morphological analysis of various bioparticles in a microfluidic channel in the future. Nature Publishing Group UK 2021-06-04 /pmc/articles/PMC8178319/ /pubmed/34088942 http://dx.doi.org/10.1038/s41598-021-91109-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Farooq, Amina Butt, Nauman Z. Hassan, Umer Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title | Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title_full | Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title_fullStr | Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title_full_unstemmed | Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title_short | Biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
title_sort | biochip with multi-planar electrodes geometry for differentiation of non-spherical bioparticles in a microchannel |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8178319/ https://www.ncbi.nlm.nih.gov/pubmed/34088942 http://dx.doi.org/10.1038/s41598-021-91109-2 |
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