Single-nucleotide resolution of N(6)-adenine methylation sites in DNA and RNA by nitrite sequencing

A single-nucleotide resolution sequencing method of N(6)-adenine methylation sites in DNA and RNA is described. Using sodium nitrite under acidic conditions, chemoselective deamination of unmethylated adenines readily occurs, without competing deamination of N(6)-adenine sites. The deamination of ad...

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Detalles Bibliográficos
Autores principales: Mahdavi-Amiri, Yasaman, Chung Kim Chung, Kimberley, Hili, Ryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2020
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179008/
https://www.ncbi.nlm.nih.gov/pubmed/34163791
http://dx.doi.org/10.1039/d0sc03509b

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179008/
https://www.ncbi.nlm.nih.gov/pubmed/34163791
http://dx.doi.org/10.1039/d0sc03509b

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