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Single-nucleotide resolution of N(6)-adenine methylation sites in DNA and RNA by nitrite sequencing
A single-nucleotide resolution sequencing method of N(6)-adenine methylation sites in DNA and RNA is described. Using sodium nitrite under acidic conditions, chemoselective deamination of unmethylated adenines readily occurs, without competing deamination of N(6)-adenine sites. The deamination of ad...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179008/ https://www.ncbi.nlm.nih.gov/pubmed/34163791 http://dx.doi.org/10.1039/d0sc03509b |