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Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry
Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179348/ https://www.ncbi.nlm.nih.gov/pubmed/34163978 http://dx.doi.org/10.1039/d0sc03889j |
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author | Strutt, Robert Hindley, James W. Gregg, Jordan Booth, Paula J. Harling, John D. Law, Robert V. Friddin, Mark S. Ces, Oscar |
author_facet | Strutt, Robert Hindley, James W. Gregg, Jordan Booth, Paula J. Harling, John D. Law, Robert V. Friddin, Mark S. Ces, Oscar |
author_sort | Strutt, Robert |
collection | PubMed |
description | Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition. |
format | Online Article Text |
id | pubmed-8179348 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-81793482021-06-22 Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry Strutt, Robert Hindley, James W. Gregg, Jordan Booth, Paula J. Harling, John D. Law, Robert V. Friddin, Mark S. Ces, Oscar Chem Sci Chemistry Droplet microcompartments linked by lipid bilayers show great promise in the construction of synthetic minimal tissues. Central to controlling the flow of information in these systems are membrane proteins, which can gate in response to specific stimuli in order to control the molecular flux between membrane separated compartments. This has been demonstrated with droplet interface bilayers (DIBs) using several different membrane proteins combined with electrical, mechanical, and/or chemical activators. Here we report the activation of the bacterial mechanosensitive channel of large conductance (MscL) in a dioleoylphosphatidylcholine:dioleoylphosphatidylglycerol DIB by controlling membrane asymmetry. We show using electrical measurements that the incorporation of lysophosphatidylcholine (LPC) into one of the bilayer leaflets triggers MscL gating in a concentration-dependent manner, with partial and full activation observed at 10 and 15 mol% LPC respectively. Our findings could inspire the design of new minimal tissues where flux pathways are dynamically defined by lipid composition. The Royal Society of Chemistry 2021-01-04 /pmc/articles/PMC8179348/ /pubmed/34163978 http://dx.doi.org/10.1039/d0sc03889j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Strutt, Robert Hindley, James W. Gregg, Jordan Booth, Paula J. Harling, John D. Law, Robert V. Friddin, Mark S. Ces, Oscar Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title | Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title_full | Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title_fullStr | Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title_full_unstemmed | Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title_short | Activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
title_sort | activating mechanosensitive channels embedded in droplet interface bilayers using membrane asymmetry |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179348/ https://www.ncbi.nlm.nih.gov/pubmed/34163978 http://dx.doi.org/10.1039/d0sc03889j |
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