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Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon

In recent years, the world has seen a troubling increase in antibiotic resistance among bacterial pathogens. In order to provide alternative strategies to combat bacterial infections, it is crucial deepen our understanding into the mechanisms that pathogens use to thrive in complex environments. Mos...

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Autores principales: Yashkin, Alex, Rayo, Josep, Grimm, Larson, Welch, Martin, Meijler, Michael M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179429/
https://www.ncbi.nlm.nih.gov/pubmed/34163722
http://dx.doi.org/10.1039/d0sc04444j
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author Yashkin, Alex
Rayo, Josep
Grimm, Larson
Welch, Martin
Meijler, Michael M.
author_facet Yashkin, Alex
Rayo, Josep
Grimm, Larson
Welch, Martin
Meijler, Michael M.
author_sort Yashkin, Alex
collection PubMed
description In recent years, the world has seen a troubling increase in antibiotic resistance among bacterial pathogens. In order to provide alternative strategies to combat bacterial infections, it is crucial deepen our understanding into the mechanisms that pathogens use to thrive in complex environments. Most bacteria use sophisticated chemical communication systems to sense their population density and coordinate gene expression in a collective manner, a process that is termed “quorum sensing” (QS). The human pathogen Pseudomonas aeruginosa uses several small molecules to regulate QS, and one of them is N-butyryl-l-homoserine lactone (C(4)-HSL). Using an activity-based protein profiling (ABPP) strategy, we designed biomimetic probes with a photoreactive group and a ‘click’ tag as an analytical handle. Using these probes, we have identified previously uncharacterized proteins that are part of the P. aeruginosa QS network, and we uncovered an additional role for this natural autoinducer in the virulence regulon of P. aeruginosa, through its interaction with PhzB1/2 that results in inhibition of pyocyanin production.
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spelling pubmed-81794292021-06-22 Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon Yashkin, Alex Rayo, Josep Grimm, Larson Welch, Martin Meijler, Michael M. Chem Sci Chemistry In recent years, the world has seen a troubling increase in antibiotic resistance among bacterial pathogens. In order to provide alternative strategies to combat bacterial infections, it is crucial deepen our understanding into the mechanisms that pathogens use to thrive in complex environments. Most bacteria use sophisticated chemical communication systems to sense their population density and coordinate gene expression in a collective manner, a process that is termed “quorum sensing” (QS). The human pathogen Pseudomonas aeruginosa uses several small molecules to regulate QS, and one of them is N-butyryl-l-homoserine lactone (C(4)-HSL). Using an activity-based protein profiling (ABPP) strategy, we designed biomimetic probes with a photoreactive group and a ‘click’ tag as an analytical handle. Using these probes, we have identified previously uncharacterized proteins that are part of the P. aeruginosa QS network, and we uncovered an additional role for this natural autoinducer in the virulence regulon of P. aeruginosa, through its interaction with PhzB1/2 that results in inhibition of pyocyanin production. The Royal Society of Chemistry 2021-01-28 /pmc/articles/PMC8179429/ /pubmed/34163722 http://dx.doi.org/10.1039/d0sc04444j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Yashkin, Alex
Rayo, Josep
Grimm, Larson
Welch, Martin
Meijler, Michael M.
Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title_full Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title_fullStr Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title_full_unstemmed Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title_short Short-chain reactive probes as tools to unravel the Pseudomonas aeruginosa quorum sensing regulon
title_sort short-chain reactive probes as tools to unravel the pseudomonas aeruginosa quorum sensing regulon
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179429/
https://www.ncbi.nlm.nih.gov/pubmed/34163722
http://dx.doi.org/10.1039/d0sc04444j
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