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Electrogenerated chemiluminescence detection of single entities

Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout;...

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Detalles Bibliográficos
Autores principales: Zhao, Wei, Chen, Hong-Yuan, Xu, Jing-Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179668/
https://www.ncbi.nlm.nih.gov/pubmed/34168801
http://dx.doi.org/10.1039/d0sc07085h
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author Zhao, Wei
Chen, Hong-Yuan
Xu, Jing-Juan
author_facet Zhao, Wei
Chen, Hong-Yuan
Xu, Jing-Juan
author_sort Zhao, Wei
collection PubMed
description Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL is an outstanding tool in analytical sensing. The traditional ECL approach measures averaged electrochemical quantities of a large ensemble of individual entities, including molecules, microstructures and ions. However, as a real system is usually heterogeneous, the study of single entities holds great potential in elucidating new truths of nature which are averaged out in ensemble assays or hidden in complex systems. We would like to review the development of ECL intensity and imaging based single entity detection and place emphasis on the assays of small entities including single molecules, micro/nanoparticles and cells. The current challenges for and perspectives on ECL detection of single entities are also discussed.
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spelling pubmed-81796682021-06-23 Electrogenerated chemiluminescence detection of single entities Zhao, Wei Chen, Hong-Yuan Xu, Jing-Juan Chem Sci Chemistry Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL is an outstanding tool in analytical sensing. The traditional ECL approach measures averaged electrochemical quantities of a large ensemble of individual entities, including molecules, microstructures and ions. However, as a real system is usually heterogeneous, the study of single entities holds great potential in elucidating new truths of nature which are averaged out in ensemble assays or hidden in complex systems. We would like to review the development of ECL intensity and imaging based single entity detection and place emphasis on the assays of small entities including single molecules, micro/nanoparticles and cells. The current challenges for and perspectives on ECL detection of single entities are also discussed. The Royal Society of Chemistry 2021-03-18 /pmc/articles/PMC8179668/ /pubmed/34168801 http://dx.doi.org/10.1039/d0sc07085h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zhao, Wei
Chen, Hong-Yuan
Xu, Jing-Juan
Electrogenerated chemiluminescence detection of single entities
title Electrogenerated chemiluminescence detection of single entities
title_full Electrogenerated chemiluminescence detection of single entities
title_fullStr Electrogenerated chemiluminescence detection of single entities
title_full_unstemmed Electrogenerated chemiluminescence detection of single entities
title_short Electrogenerated chemiluminescence detection of single entities
title_sort electrogenerated chemiluminescence detection of single entities
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179668/
https://www.ncbi.nlm.nih.gov/pubmed/34168801
http://dx.doi.org/10.1039/d0sc07085h
work_keys_str_mv AT zhaowei electrogeneratedchemiluminescencedetectionofsingleentities
AT chenhongyuan electrogeneratedchemiluminescencedetectionofsingleentities
AT xujingjuan electrogeneratedchemiluminescencedetectionofsingleentities