A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens

We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR ass...

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Autores principales: Wang, Xinye, Xiu, Leshan, Binder, Raquel A., Toh, Teck-Hock, Lee, Jeffrey Soon-Yit, Ting, Jakie, Than, Son T., Qi, Wenhao, Coleman, Kristen K., Perera, David, Ma, Maijuan, Gray, Gregory C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179717/
https://www.ncbi.nlm.nih.gov/pubmed/34124332
http://dx.doi.org/10.1016/j.onehlt.2021.100274
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author Wang, Xinye
Xiu, Leshan
Binder, Raquel A.
Toh, Teck-Hock
Lee, Jeffrey Soon-Yit
Ting, Jakie
Than, Son T.
Qi, Wenhao
Coleman, Kristen K.
Perera, David
Ma, Maijuan
Gray, Gregory C.
author_facet Wang, Xinye
Xiu, Leshan
Binder, Raquel A.
Toh, Teck-Hock
Lee, Jeffrey Soon-Yit
Ting, Jakie
Than, Son T.
Qi, Wenhao
Coleman, Kristen K.
Perera, David
Ma, Maijuan
Gray, Gregory C.
author_sort Wang, Xinye
collection PubMed
description We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.
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spelling pubmed-81797172021-06-07 A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens Wang, Xinye Xiu, Leshan Binder, Raquel A. Toh, Teck-Hock Lee, Jeffrey Soon-Yit Ting, Jakie Than, Son T. Qi, Wenhao Coleman, Kristen K. Perera, David Ma, Maijuan Gray, Gregory C. One Health Research Paper We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations. Elsevier 2021-06-06 /pmc/articles/PMC8179717/ /pubmed/34124332 http://dx.doi.org/10.1016/j.onehlt.2021.100274 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Wang, Xinye
Xiu, Leshan
Binder, Raquel A.
Toh, Teck-Hock
Lee, Jeffrey Soon-Yit
Ting, Jakie
Than, Son T.
Qi, Wenhao
Coleman, Kristen K.
Perera, David
Ma, Maijuan
Gray, Gregory C.
A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title_full A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title_fullStr A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title_full_unstemmed A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title_short A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens
title_sort pan-coronavirus rt-pcr assay for rapid viral screening of animal, human, and environmental specimens
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179717/
https://www.ncbi.nlm.nih.gov/pubmed/34124332
http://dx.doi.org/10.1016/j.onehlt.2021.100274
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