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Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique

Colorectal cancer (CRC) is a major global public health problem. Folate metabolism is involved in DNA synthesis, repair and methylation. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism. Common MTHFR C677T polymorphism was correlated to CRC. This case-control study wa...

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Autores principales: Baghad, Imane, Erreguibi, Driss, Boufettal, Rachid, Eljai, Saad Rifki, Chihab, Farid, Nadifi, Sellama
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The African Field Epidemiology Network 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179987/
https://www.ncbi.nlm.nih.gov/pubmed/34122714
http://dx.doi.org/10.11604/pamj.2021.38.287.12522
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author Baghad, Imane
Erreguibi, Driss
Boufettal, Rachid
Eljai, Saad Rifki
Chihab, Farid
Nadifi, Sellama
author_facet Baghad, Imane
Erreguibi, Driss
Boufettal, Rachid
Eljai, Saad Rifki
Chihab, Farid
Nadifi, Sellama
author_sort Baghad, Imane
collection PubMed
description Colorectal cancer (CRC) is a major global public health problem. Folate metabolism is involved in DNA synthesis, repair and methylation. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism. Common MTHFR C677T polymorphism was correlated to CRC. This case-control study was conducted to analyze the association between this polymorphism and the risk of sporadic CRC in a Moroccan population. The study involved 76 patients with sporadic colorectal cancer confirmed histologically and 182 patients (control group) without a history of cancer. Deoxyribonucleic acid (DNA) was isolated from peripheral blood and genotypes were determined using PCR-RFLP. The risk of association was estimated using odds ratio (OR) with 95% confidence interval. Genotype frequency of MTHFR in patients and in the control group was CC 34.1%, CT 56.6%, TT 9.21%, CC 51.6%, CT 42.8% and TT 6% respectively. CT genotype and its combination with TT genotype and allele T were associated with an increased risk of CRC and with an OR of 2.02 (with 95% confidence interval [CI]: 1.14-3.58, p = 0.01), 2.05 (95 % CI: 1.18-3.58, p= 0.01) and 1.61 (95% CI: 1.07-2.40, p=0.02). Homozygous TT weren´t a protection factor in our study, with an OR of 2.30 (95% CI: 0.81-6.52, p = 0.11). There was a statistically significant association between the MTHFR C677T variant and the risk of occurrence of sporadic colorectal cancer in the studied population.
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spelling pubmed-81799872021-06-11 Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique Baghad, Imane Erreguibi, Driss Boufettal, Rachid Eljai, Saad Rifki Chihab, Farid Nadifi, Sellama Pan Afr Med J Case Series Colorectal cancer (CRC) is a major global public health problem. Folate metabolism is involved in DNA synthesis, repair and methylation. Methylenetetrahydrofolate reductase (MTHFR) is a key enzyme in folate metabolism. Common MTHFR C677T polymorphism was correlated to CRC. This case-control study was conducted to analyze the association between this polymorphism and the risk of sporadic CRC in a Moroccan population. The study involved 76 patients with sporadic colorectal cancer confirmed histologically and 182 patients (control group) without a history of cancer. Deoxyribonucleic acid (DNA) was isolated from peripheral blood and genotypes were determined using PCR-RFLP. The risk of association was estimated using odds ratio (OR) with 95% confidence interval. Genotype frequency of MTHFR in patients and in the control group was CC 34.1%, CT 56.6%, TT 9.21%, CC 51.6%, CT 42.8% and TT 6% respectively. CT genotype and its combination with TT genotype and allele T were associated with an increased risk of CRC and with an OR of 2.02 (with 95% confidence interval [CI]: 1.14-3.58, p = 0.01), 2.05 (95 % CI: 1.18-3.58, p= 0.01) and 1.61 (95% CI: 1.07-2.40, p=0.02). Homozygous TT weren´t a protection factor in our study, with an OR of 2.30 (95% CI: 0.81-6.52, p = 0.11). There was a statistically significant association between the MTHFR C677T variant and the risk of occurrence of sporadic colorectal cancer in the studied population. The African Field Epidemiology Network 2021-03-18 /pmc/articles/PMC8179987/ /pubmed/34122714 http://dx.doi.org/10.11604/pamj.2021.38.287.12522 Text en Copyright: Imane Baghad et al. https://creativecommons.org/licenses/by/4.0/The Pan African Medical Journal (ISSN: 1937-8688). This is an Open Access article distributed under the terms of the Creative Commons Attribution International 4.0 License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Case Series
Baghad, Imane
Erreguibi, Driss
Boufettal, Rachid
Eljai, Saad Rifki
Chihab, Farid
Nadifi, Sellama
Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title_full Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title_fullStr Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title_full_unstemmed Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title_short Association du polymorphisme de la méthylènetétrahydrofolate réductase C677T avec le risque de cancer colorectal sporadique
title_sort association du polymorphisme de la méthylènetétrahydrofolate réductase c677t avec le risque de cancer colorectal sporadique
topic Case Series
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8179987/
https://www.ncbi.nlm.nih.gov/pubmed/34122714
http://dx.doi.org/10.11604/pamj.2021.38.287.12522
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