LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis

BACKGROUND: The incidence of gallbladder carcinoma (GBM) in China has increased in recent years. Here, the functional mechanism of lncRNA TTN-AS1 in GBC was preliminary elucidated. METHODS: The expression levels of lncRNA TTN-AS1, miR-107, and HMGA1 in tissues and cell lines were assessed by RT-qPCR...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Zhaoxia, Li, Yaosheng, Shao, Rongfeng, Hu, Yuqing, Gao, Han
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180155/
https://www.ncbi.nlm.nih.gov/pubmed/34090483
http://dx.doi.org/10.1186/s12957-021-02279-2
_version_ 1783703941237702656
author Lin, Zhaoxia
Li, Yaosheng
Shao, Rongfeng
Hu, Yuqing
Gao, Han
author_facet Lin, Zhaoxia
Li, Yaosheng
Shao, Rongfeng
Hu, Yuqing
Gao, Han
author_sort Lin, Zhaoxia
collection PubMed
description BACKGROUND: The incidence of gallbladder carcinoma (GBM) in China has increased in recent years. Here, the functional mechanism of lncRNA TTN-AS1 in GBC was preliminary elucidated. METHODS: The expression levels of lncRNA TTN-AS1, miR-107, and HMGA1 in tissues and cell lines were assessed by RT-qPCR. Cell proliferation was measured by MTT assays. Cell invasion and migration abilities were evaluated by Transwell assays. The relationship between miR-107 and lncRNA TTN-AS1 or HMGA1 was confirmed by luciferase reporter assay. RESULTS: Upregulation of lncRNA TTN-AS1 and downregulation of miR-107 were detected in GBC. Furthermore, the expressions between TTN-AS1 and miR-107 were mutually inhibited in GBC. Functionally, lncRNA TTN-AS1 promoted cell viability and motility in GBC by sponging miR-107. In addition, miR-107 directly targets HMGA1. And HMGA1 can be positively regulated by lncRNA TTN-AS1 in GBC. Furthermore, HMGA1 promoted GBC progression by interacting with lncRNA TTN-AS1/miR-107 axis. CONCLUSION: LncRNA TTN-AS1 acted as a tumor promoter in GBC by sponging miR-107 and upregulating HMGA1.
format Online
Article
Text
id pubmed-8180155
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-81801552021-06-07 LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis Lin, Zhaoxia Li, Yaosheng Shao, Rongfeng Hu, Yuqing Gao, Han World J Surg Oncol Research BACKGROUND: The incidence of gallbladder carcinoma (GBM) in China has increased in recent years. Here, the functional mechanism of lncRNA TTN-AS1 in GBC was preliminary elucidated. METHODS: The expression levels of lncRNA TTN-AS1, miR-107, and HMGA1 in tissues and cell lines were assessed by RT-qPCR. Cell proliferation was measured by MTT assays. Cell invasion and migration abilities were evaluated by Transwell assays. The relationship between miR-107 and lncRNA TTN-AS1 or HMGA1 was confirmed by luciferase reporter assay. RESULTS: Upregulation of lncRNA TTN-AS1 and downregulation of miR-107 were detected in GBC. Furthermore, the expressions between TTN-AS1 and miR-107 were mutually inhibited in GBC. Functionally, lncRNA TTN-AS1 promoted cell viability and motility in GBC by sponging miR-107. In addition, miR-107 directly targets HMGA1. And HMGA1 can be positively regulated by lncRNA TTN-AS1 in GBC. Furthermore, HMGA1 promoted GBC progression by interacting with lncRNA TTN-AS1/miR-107 axis. CONCLUSION: LncRNA TTN-AS1 acted as a tumor promoter in GBC by sponging miR-107 and upregulating HMGA1. BioMed Central 2021-06-05 /pmc/articles/PMC8180155/ /pubmed/34090483 http://dx.doi.org/10.1186/s12957-021-02279-2 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lin, Zhaoxia
Li, Yaosheng
Shao, Rongfeng
Hu, Yuqing
Gao, Han
LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title_full LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title_fullStr LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title_full_unstemmed LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title_short LncRNA TTN-AS1 acts as a tumor promoter in gallbladder carcinoma by regulating miR-107/HMGA1 axis
title_sort lncrna ttn-as1 acts as a tumor promoter in gallbladder carcinoma by regulating mir-107/hmga1 axis
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180155/
https://www.ncbi.nlm.nih.gov/pubmed/34090483
http://dx.doi.org/10.1186/s12957-021-02279-2
work_keys_str_mv AT linzhaoxia lncrnattnas1actsasatumorpromoteringallbladdercarcinomabyregulatingmir107hmga1axis
AT liyaosheng lncrnattnas1actsasatumorpromoteringallbladdercarcinomabyregulatingmir107hmga1axis
AT shaorongfeng lncrnattnas1actsasatumorpromoteringallbladdercarcinomabyregulatingmir107hmga1axis
AT huyuqing lncrnattnas1actsasatumorpromoteringallbladdercarcinomabyregulatingmir107hmga1axis
AT gaohan lncrnattnas1actsasatumorpromoteringallbladdercarcinomabyregulatingmir107hmga1axis

Ejemplares similares