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Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages
BACKGROUND: Differences in immune responses against different lineages of Mycobacterium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune re...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180191/ https://www.ncbi.nlm.nih.gov/pubmed/34141493 http://dx.doi.org/10.7717/peerj.11565 |
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author | Kaewseekhao, Benjawan Roytrakul, Sittiruk Yingchutrakul, Yodying Laohaviroj, Marut Salao, Kanin Faksri, Kiatichai |
author_facet | Kaewseekhao, Benjawan Roytrakul, Sittiruk Yingchutrakul, Yodying Laohaviroj, Marut Salao, Kanin Faksri, Kiatichai |
author_sort | Kaewseekhao, Benjawan |
collection | PubMed |
description | BACKGROUND: Differences in immune responses against different lineages of Mycobacterium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune responses were also investigated during infection and when the bacilli had been eliminated from the immune cells. METHODS: Human primary leukocytes were infected with strains representing three lineages of Mtb (East-Asian, Indo-Oceanic and Euro-American). Label-free GeLC MS/MS proteomic analysis of secretomes was performed. The response of each immune-cell type was compared with the appropriate interactome database for each. RESULTS: The expression pattern of proteins secreted by Mtb-infected leukocytes differed among Mtb lineages. The ancestral lineage (IO lineage) had a greater ability to activate MMP14 (associated with leukocyte migration) than did the more recent lineages (EA and EuA). During infection, proteins secreted by macrophages, dendritic cells, neutrophils and B-cells were associated with cell proliferation. Following clearance of Mtb, proteins associated with interferon signaling were found in macrophages, dendritic cells and neutrophils: proteins associated with antigen processing were found in B-cells and regulatory T-cells. Expression of immune response-related proteins from many immune-cell types might be suppressed by Mtb infection. Our study has provided a better insight into the host-pathogen interaction and immune response against different Mtb lineages. |
format | Online Article Text |
id | pubmed-8180191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81801912021-06-16 Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages Kaewseekhao, Benjawan Roytrakul, Sittiruk Yingchutrakul, Yodying Laohaviroj, Marut Salao, Kanin Faksri, Kiatichai PeerJ Biodiversity BACKGROUND: Differences in immune responses against different lineages of Mycobacterium tuberculosis (Mtb), and by different types of immune cell, are still poorly understood. We aimed to compare the secretome-based immune responses among three Mtb lineages and among immune-cell types. The immune responses were also investigated during infection and when the bacilli had been eliminated from the immune cells. METHODS: Human primary leukocytes were infected with strains representing three lineages of Mtb (East-Asian, Indo-Oceanic and Euro-American). Label-free GeLC MS/MS proteomic analysis of secretomes was performed. The response of each immune-cell type was compared with the appropriate interactome database for each. RESULTS: The expression pattern of proteins secreted by Mtb-infected leukocytes differed among Mtb lineages. The ancestral lineage (IO lineage) had a greater ability to activate MMP14 (associated with leukocyte migration) than did the more recent lineages (EA and EuA). During infection, proteins secreted by macrophages, dendritic cells, neutrophils and B-cells were associated with cell proliferation. Following clearance of Mtb, proteins associated with interferon signaling were found in macrophages, dendritic cells and neutrophils: proteins associated with antigen processing were found in B-cells and regulatory T-cells. Expression of immune response-related proteins from many immune-cell types might be suppressed by Mtb infection. Our study has provided a better insight into the host-pathogen interaction and immune response against different Mtb lineages. PeerJ Inc. 2021-06-03 /pmc/articles/PMC8180191/ /pubmed/34141493 http://dx.doi.org/10.7717/peerj.11565 Text en ©2021 Kaewseekhao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Biodiversity Kaewseekhao, Benjawan Roytrakul, Sittiruk Yingchutrakul, Yodying Laohaviroj, Marut Salao, Kanin Faksri, Kiatichai Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title | Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title_full | Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title_fullStr | Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title_full_unstemmed | Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title_short | Characterisation of secretome-based immune responses of human leukocytes infected with various Mycobacterium tuberculosis lineages |
title_sort | characterisation of secretome-based immune responses of human leukocytes infected with various mycobacterium tuberculosis lineages |
topic | Biodiversity |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8180191/ https://www.ncbi.nlm.nih.gov/pubmed/34141493 http://dx.doi.org/10.7717/peerj.11565 |
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