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Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors

Advanced stage glioma is the most aggressive form of malignant brain tumors with a short survival time. Real-time pathology assisted, or image guided surgical procedures that eliminate tumors promise to improve the clinical outcome and prolong the lives of patients. Our work is focused on the develo...

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Autores principales: Lukina, Maria, Yashin, Konstantin, Kiseleva, Elena E., Alekseeva, Anna, Dudenkova, Varvara, Zagaynova, Elena V., Bederina, Evgenia, Medyanic, Igor, Becker, Wolfgang, Mishra, Deependra, Berezin, Mikhail, Shcheslavskiy, Vladislav I., Shirmanova, Marina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181388/
https://www.ncbi.nlm.nih.gov/pubmed/34109119
http://dx.doi.org/10.3389/fonc.2021.666059
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author Lukina, Maria
Yashin, Konstantin
Kiseleva, Elena E.
Alekseeva, Anna
Dudenkova, Varvara
Zagaynova, Elena V.
Bederina, Evgenia
Medyanic, Igor
Becker, Wolfgang
Mishra, Deependra
Berezin, Mikhail
Shcheslavskiy, Vladislav I.
Shirmanova, Marina
author_facet Lukina, Maria
Yashin, Konstantin
Kiseleva, Elena E.
Alekseeva, Anna
Dudenkova, Varvara
Zagaynova, Elena V.
Bederina, Evgenia
Medyanic, Igor
Becker, Wolfgang
Mishra, Deependra
Berezin, Mikhail
Shcheslavskiy, Vladislav I.
Shirmanova, Marina
author_sort Lukina, Maria
collection PubMed
description Advanced stage glioma is the most aggressive form of malignant brain tumors with a short survival time. Real-time pathology assisted, or image guided surgical procedures that eliminate tumors promise to improve the clinical outcome and prolong the lives of patients. Our work is focused on the development of a rapid and sensitive assay for intraoperative diagnostics of glioma and identification of optical markers essential for differentiation between tumors and healthy brain tissues. We utilized fluorescence lifetime imaging (FLIM) of endogenous fluorophores related to metabolism of the glioma from freshly excised brains tissues. Macroscopic time-resolved fluorescence images of three intracranial animal glioma models and surgical samples of patients’ glioblastoma together with the white matter have been collected. Several established and new algorithms were applied to identify the imaging markers of the tumors. We found that fluorescence lifetime parameters characteristic of the glioma provided background for differentiation between the tumors and intact brain tissues. All three rat tumor models demonstrated substantial differences between the malignant and normal tissue. Similarly, tumors from patients demonstrated statistically significant differences from the peritumoral white matter without infiltration. While the data and the analysis presented in this paper are preliminary and further investigation with a larger number of samples is required, the proposed approach based on the macroscopic FLIM has a high potential for diagnostics of glioma and evaluation of the surgical margins of gliomas.
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spelling pubmed-81813882021-06-08 Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors Lukina, Maria Yashin, Konstantin Kiseleva, Elena E. Alekseeva, Anna Dudenkova, Varvara Zagaynova, Elena V. Bederina, Evgenia Medyanic, Igor Becker, Wolfgang Mishra, Deependra Berezin, Mikhail Shcheslavskiy, Vladislav I. Shirmanova, Marina Front Oncol Oncology Advanced stage glioma is the most aggressive form of malignant brain tumors with a short survival time. Real-time pathology assisted, or image guided surgical procedures that eliminate tumors promise to improve the clinical outcome and prolong the lives of patients. Our work is focused on the development of a rapid and sensitive assay for intraoperative diagnostics of glioma and identification of optical markers essential for differentiation between tumors and healthy brain tissues. We utilized fluorescence lifetime imaging (FLIM) of endogenous fluorophores related to metabolism of the glioma from freshly excised brains tissues. Macroscopic time-resolved fluorescence images of three intracranial animal glioma models and surgical samples of patients’ glioblastoma together with the white matter have been collected. Several established and new algorithms were applied to identify the imaging markers of the tumors. We found that fluorescence lifetime parameters characteristic of the glioma provided background for differentiation between the tumors and intact brain tissues. All three rat tumor models demonstrated substantial differences between the malignant and normal tissue. Similarly, tumors from patients demonstrated statistically significant differences from the peritumoral white matter without infiltration. While the data and the analysis presented in this paper are preliminary and further investigation with a larger number of samples is required, the proposed approach based on the macroscopic FLIM has a high potential for diagnostics of glioma and evaluation of the surgical margins of gliomas. Frontiers Media S.A. 2021-05-24 /pmc/articles/PMC8181388/ /pubmed/34109119 http://dx.doi.org/10.3389/fonc.2021.666059 Text en Copyright © 2021 Lukina, Yashin, Kiseleva, Alekseeva, Dudenkova, Zagaynova, Bederina, Medyanic, Becker, Mishra, Berezin, Shcheslavskiy and Shirmanova https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Lukina, Maria
Yashin, Konstantin
Kiseleva, Elena E.
Alekseeva, Anna
Dudenkova, Varvara
Zagaynova, Elena V.
Bederina, Evgenia
Medyanic, Igor
Becker, Wolfgang
Mishra, Deependra
Berezin, Mikhail
Shcheslavskiy, Vladislav I.
Shirmanova, Marina
Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title_full Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title_fullStr Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title_full_unstemmed Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title_short Label-Free Macroscopic Fluorescence Lifetime Imaging of Brain Tumors
title_sort label-free macroscopic fluorescence lifetime imaging of brain tumors
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8181388/
https://www.ncbi.nlm.nih.gov/pubmed/34109119
http://dx.doi.org/10.3389/fonc.2021.666059
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