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Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids

Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluorescence...

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Detalles Bibliográficos
Autores principales: Bergdorf, Kensey N., Phifer, Courtney J., Bechard, Matthew E., Lee, Mason A., McDonald, Oliver G., Lee, Ethan, Weiss, Vivian L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182106/
https://www.ncbi.nlm.nih.gov/pubmed/34136836
http://dx.doi.org/10.1016/j.xpro.2021.100578
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author Bergdorf, Kensey N.
Phifer, Courtney J.
Bechard, Matthew E.
Lee, Mason A.
McDonald, Oliver G.
Lee, Ethan
Weiss, Vivian L.
author_facet Bergdorf, Kensey N.
Phifer, Courtney J.
Bechard, Matthew E.
Lee, Mason A.
McDonald, Oliver G.
Lee, Ethan
Weiss, Vivian L.
author_sort Bergdorf, Kensey N.
collection PubMed
description Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluorescence is a powerful tool to help understand the localization of cell types within organoids and determine the interactions between those cells. As organoids have been shown to recapitulate patient tumor morphology, immunofluorescent staining and imaging of organoids allows for enhanced understanding of near in vivo structures. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020).
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spelling pubmed-81821062021-06-15 Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids Bergdorf, Kensey N. Phifer, Courtney J. Bechard, Matthew E. Lee, Mason A. McDonald, Oliver G. Lee, Ethan Weiss, Vivian L. STAR Protoc Protocol Our organoid generation technique has allowed for the development of downstream organoid applications. Here, we detail an accessible, straightforward protocol for immunofluorescent staining and imaging of thyroid cancer organoids, particularly those with tumor de-differentiation. Immunofluorescence is a powerful tool to help understand the localization of cell types within organoids and determine the interactions between those cells. As organoids have been shown to recapitulate patient tumor morphology, immunofluorescent staining and imaging of organoids allows for enhanced understanding of near in vivo structures. For complete details on the use and execution of this protocol, please refer to Lee et al. (2020) and Vilgelm et al. (2020). Elsevier 2021-05-31 /pmc/articles/PMC8182106/ /pubmed/34136836 http://dx.doi.org/10.1016/j.xpro.2021.100578 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Bergdorf, Kensey N.
Phifer, Courtney J.
Bechard, Matthew E.
Lee, Mason A.
McDonald, Oliver G.
Lee, Ethan
Weiss, Vivian L.
Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title_full Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title_fullStr Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title_full_unstemmed Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title_short Immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
title_sort immunofluorescent staining of cancer spheroids and fine-needle aspiration-derived organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8182106/
https://www.ncbi.nlm.nih.gov/pubmed/34136836
http://dx.doi.org/10.1016/j.xpro.2021.100578
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